The objective of this work was to increase laccase production by Pleurotus ostreatus PLAB through culture medium optimization using solid-state culture conditions. Increased laccase activity was obtained through the design of experiments (DOE) using the Taguchi orthogonal array (OA). Seven factors, viz. lignocellulose, glucose, yeast extract, peptone, KH 2 PO 4 , MgSO 4 · 7H 2 O and MnSO 4 · H 2 O at three levels and pH at two levels with OA layout of L18 (2 1 × 3 7 ) were selected for the proposed experimental design using Minitab 17 software. Data analysis showed that lignocellulose (20%) and glucose (10 g L were the optimal conditions to maximize laccase production. The model predicted a 30.37 U g -1 dry wt., which agreed with the experimentally obtained laccase activity 29.15 U g -1 dry wt. at optimal conditions.
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