Effect of Solid State Fermentation Medium Optimization on Pleurotus ostreatus Laccase Production

Abstract

The objective of this work was to increase laccase production by Pleurotus ostreatus PLAB through culture medium optimization using solid-state culture conditions. Increased laccase activity was obtained through the design of experiments (DOE) using the Taguchi orthogonal array (OA). Seven factors, viz. lignocellulose, glucose, yeast extract, peptone, KH 2 PO 4 , MgSO 4 · 7H 2 O and MnSO 4 · H 2 O at three levels and pH at two levels with OA layout of L18 (2 1 × 3 7 ) were selected for the proposed experimental design using Minitab 17 software. Data analysis showed that lignocellulose (20%) and glucose (10 g L were the optimal conditions to maximize laccase production. The model predicted a 30.37 U g -1 dry wt., which agreed with the experimentally obtained laccase activity 29.15 U g -1 dry wt. at optimal conditions.

3rd International Conference on Technologies & Business Models for Circular Economy: Conference Proceedings

“…Enzymatic activity was highest on substrates that contained bran, had high nitrogen content and low C/N ratio (Kurt & Buyukalaca, 2010). Belšak-Šel et al (Belšak-Šel et al, 2015) studied the increase in laccase production from P. ostreatus by optimizing the culture medium. The level of laccase activity produced by P.…”