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Introduction: Many widespread intractable diseases are caused or supported by chronic inflammation. Such conditions include the 2nd type of diabetes mellitus, atherosclerosis, neurodegenerative diseases, chronic inflammatory diseases of the connective tissue-ankylosing spondylitis, rheumatoid arthritis, autoimmune myositis, etc. Therefore, the search for targeted treatment of these illnesses is extremely in high demand. Immunomodulatory activity of mesenchymal stem cells is one of their remarkable properties. Several biomarkers (cytokines and nonspecific proteins) are known to be associated with chronic inflammation. Methods: Our study aimed to investigate the serum levels of tumor necrosis factor-alpha, interleukin 6 and C-reactive protein in carrageenan myositis in rats, because there is more and more evidence of the significance of these markers in the course and resolution of the diseases mentioned above. For the first time in our experiment, it was shown and evaluated using analysis of variance, how MSCs influence the indicators of proinflammatory cytokines on the model of carrageenan myositis. The levels of α-TNF, IL-6 and CRP in the plasma of rats were studied in groups with chronic carrageenan inflammation and chronic inflammation with local injection of MSCs into the affected area. Results: Our study proved the effectiveness of MSCs by showing a significant decrease in the levels of inflammatory mediators in the plasma of the studied animals. Discussion and Conclusions: Thus, the administration of MSCs is a promising tool in the pathogenic treatment of chronic inflammation and concomitant conditions.

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The chronic inflammatory process is a pathological condition characterized by an ongoing active inflammatory response and tissue destruction. Many studies show that chronic inflammation can play a severe role in various age-related diseases, including diabetes, cardiovascular, and autoimmune diseases. One of the important but poorly studied factors affecting the regulation of chronic inflammation is the regulatory activity of MSCs. In this regard, the study of mesenchymal stem cells preventing chronic inflammation in the experiment is an important area of modern pathology. On the one hand, increased cytokines, such as α-TNF, IL 6, and CRP, are reliable tools in diagnostic different inflammatory processes, especially chronic inflammation. On the other hand, nowadays, we need a more straightforward and not so expensive criterion for this purpose, for instance, a common total blood count and LMR. For the first time, we investigated how trustworthy can be LMR and how possible to use it in chronic inflammation in rats to achieve prognostic goals.  This study investigated the correlation between α-TNF, IL-6, and CRP with LMR in rats' plasma in groups with chronic carrageenan inflammation and chronic inflammation with local injection of MSCs into the affected area. The study involved 132 adult male rats (180-220g), which were divided into groups. The inflammation model was chronic aseptic myositis caused by an intramuscular injection of 10mg λ-carrageenan (Sigma-Aldrich GmbH). Our experimental groups of rats were treated with MSCs (the injection into the inflamed site) in the amount of 1-2 million cells once. Blood sampling was performed from 6 hours to 28 days. We calculated our results using Statistica (data analysis software) version 13. For comparison, we used one-way ANOVA, Turkey's post hoc test, where p <0.05 was considered statistically significant.  In our experiment, the correlation between levels of α-TNF, IL-6, and CRP with lymphocyte-monocyte ratio in rats was described for the first time, demonstrating the suppression of chronic inflammation through MSCs.

Medical doctors may not have the necessary evidence-based knowledge of specific leadership styles to excel in a leadership role. This article explores the various leadership styles adopted by a clinical professional transitioning into a leadership role. The goal-path theory, developed by Robert House in 1971, was used as the theoretical lens for this study. Twenty peer-reviewed scientific articles, written in English and published between 2015 and 2020, were analyzed and synthesized to produce results. The results showed that employee retention was positively associated with transformational and authentic leadership styles; organizational commitment was positively related to transformational, transactional, and genuine leadership styles; and job satisfaction was positively related to transformational and authentic leadership styles. In particular, the transformational leadership style showed higher employee retention and job satisfaction rates than the transactional and non-interventional leadership styles. The original and transformative leadership styles increased job satisfaction and commitment, but the correlation between an authentic leadership style and these benefits was less clear. Clinical professionals must apply a transformational leadership style to become influential leaders.