Buah kopi termasuk buah musiman yang hanya muncul pada bulan bulan tertentu. Di daerah Gayo kopi merupakan minuman yang hampir setiap hari di konsumsi. Kopi diduga mempunyai aktivitas antioksidan sehingga mampu merendam aktivitas radikal bebas. Tujuan dari penelitian ini melakukan skrining fitokimia dan mengetahui aktivitas antioksidan dari ekstrak etanol biji kopi arabika dengan metoda 2,2-Diphenyl-2-picrylhydrazyl (DPPH). Ekstrak biji kopi arabika di buat dengan cara perkolasi dengan menggunakan pelarut etanol dan ekstrak yang diperoleh di pekatkan menggunakan rotary evaporator, selanjutnya ekstrak etanol di uji kekuatan aktifitas antioksidannya dengan menggunakan metoda 2,2-Diphenyl-2-picrylhydrazyl (DPPH) pada panjang gelombang maksimum 516 nm, sebagai kontrol positif digunakan vitamin C. Hasil skrining fitokimia menunjukkan bahwa ekstrak etanol biji kopi arabika mengandung golongan senyawa tanin, alkaloid, saponin, flavonoid, dan steroid. Hasil penelitian menunjukkan aktivitas antioksidan ekstrak etanol biji kopi arabika termasuk kategori sangat kuat dengan IC50 12,427 ppm dan vitamin C dengan IC50 0,273 ppm. Kata kunci : Skrining Fitokimia, Antioksidan, Ekstrak Biji Kopi Arabika, DPPH.
Background: Castanopsis costata (Blume) A. DC. or commonly known as cep-cepan is a medicinal plant widely used based on experience by Karo people in Indonesia n the Karo community for the treatment of wounds. However, no research has been done to prove this experience. Initially, the formulation of ointment extract and leaf fractions of C. costata need to be carried out. Aim: The purpose of this study was to provide information about the chemical group content contained in C. costata leaf extract and fractions and to evaluate their ointment preparation according to the requirements of ointment preparations. Method: The extraction of C. costata leaves using 70% ethanol using the maceration method followed by the fractionation of C. costata leaves using water, ethyl acetate, and n-hexane as solvents and phytochemical screening were carried out. Furthermore, the preparation of ointments and evaluation of extract ointment preparations and C. costata leaf fraction ointment was performed. Result: The results indicated that the ethanol extract of C. costata contained alkaloids, flavonoids, glycosides, steroids/terpenoids, saponins and tannins, while the water fraction contained flavonoids, saponins and tannins. The ethyl acetate fraction contained flavonoids, steroids/terpenoids, saponins, and tannins and the n-hexane fraction contained alkaloids and steroids/terpenoids. The results also indicated that the extract and fraction ointment preparations and ointment fractions evaluation, including organoleptic, homogeneity, and pH, met the evaluation requirements for ointment. Conclusion: Ethanol extract of C. costata contains alkaloids, flavonoids, glycosides, steroids/terpenoids, saponins and tannins, while the water fraction contains flavonoids, saponins and tannins. The ethyl acetate fraction contains flavonoids, steroids/terpenoids, saponins, and tannins and the n-hexane fraction contains alkaloids and steroids/terpenoids. The extract and fraction ointment preparations and ointment met the evaluation requirements for ointment.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.