Objective: To develop simple, accurate, precise UV Spectrophotometric method for the simultaneous estimation of Sitagliptin and Metformin in tablet dosage form.
Methodols: The method is based on the determination of Sitagliptin and Metformin in tablet using simultaneous equation method. Sitagliptin exhibits maximum absorbance at 267 and Metformin exhibits maximum absorbance at 237 nm using distilled water as diluents.
Results: The calibration curve was linear in the range of 10-300 µg/ml for Sitagliptin and 4-14µg/ml for Metformin. The %RSD were within the limit i.e., less than 2%. The % recovery of the proposed method was found to be 97.12-99.46% for Sitagliptin and 98.15-99.85% for Metformin. The LOD of the proposed method was 0.397μg/ml for Sitagliptin and 0.8952µg/ml for Metformin. The LOQ was 1.2951μg/ml for Sitagliptin and 2.7159μg/ml for Metformin.
Conclusion: A simple, accurate, precise UV Spectrophotometric method for the simultaneous estimation of Sitagliptin and Metformin in tablet dosage form.
Aims: To develop and validate a new, simple, rapid, precise and accurate Reverse Phase High Performance Liquid Chromatographic (RP-HPLC) method for the quantitative determination of Tigecycline in bulk and pharmaceutical dosage form.
Study Design:
Place and Duration of the Study: RBVRR women's college of pharmacy, Barkatpura, Hyderabad, between june 2019 and july 2020.
Methodology: The RP-HPLC method was developed on Sunsil C18 150 mm x 4.6mm x 5µ column using acetonitrile : water (pH maintained at 3.5 with acetic acid) [70:30] as mobile phase at flow rate 0.8 ml/min and UV detection at 250 nm.
Results: Tigecycline exhibited linearity over the concentration range of 5-40 µg/mL (R2 > 0.999). The analytical method showed good precision with % RSD below 2. The method showed suitable accuracy and robustness.
Conclusion: Validation of the developed method was done as per International Conference on Harmonization (ICH) Q2R1 guidelines.
Tolvaptan in API and formulation may now be quantifi ed using a technique. The solution was diluted with acetonitrile and scanned in the UV area between 200 and 400 nm. At 267 nm, tolvaptan exhibits maximum absorption. The accuracy investigations had been executed at3 levels, i.e., 80, 100, and 120%, and recuperation turned into discovered to bewith inside the variety of 99.4% for the tolvaptan, which showed linearity over the range of 5 to 160 g/mL with correlation co-effi cient (r2) of 0.9995. The quantifi cation (LoQ) and detection (LoD) threshold were 0.471 and 1.435 g/mL, respectively. The suggested approach underwent forced deterioration, and each parameter’s degradation was discovered. The ICH rules were followed in the validation of each parameter.
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