Accurately 10 mg of Tigecycline pure drug was weighed and transferred into a 10 ml volumetric flask. The volume was made up to the mark using Acetonitrile (ACN) resulting in 1000 μg/ml concentration primary stock solution. From this 1 ml aliquot was transferred into a 10 ml volumetric
Aims: To develop and validate a new, simple, rapid, precise and accurate Reverse Phase High Performance Liquid Chromatographic (RP-HPLC) method for the quantitative determination of Tigecycline in bulk and pharmaceutical dosage form.
Study Design:
Place and Duration of the Study: RBVRR women's college of pharmacy, Barkatpura, Hyderabad, between june 2019 and july 2020.
Methodology: The RP-HPLC method was developed on Sunsil C18 150 mm x 4.6mm x 5µ column using acetonitrile : water (pH maintained at 3.5 with acetic acid) [70:30] as mobile phase at flow rate 0.8 ml/min and UV detection at 250 nm.
Results: Tigecycline exhibited linearity over the concentration range of 5-40 µg/mL (R2 > 0.999). The analytical method showed good precision with % RSD below 2. The method showed suitable accuracy and robustness.
Conclusion: Validation of the developed method was done as per International Conference on Harmonization (ICH) Q2R1 guidelines.
Objective:The aim of the present study is to develop and validate a simple, efficient, economical and accurate UV-visible spectrophotometric method for estimation of bosentan in spiked human plasma.
Methods:The analyte was extracted by Liquid-liquid Extraction (LLE) procedure using acetonitrile and chloroform. Absorbance of the analyte in the extract was measured at 270 nm using ethanol as a diluent. The developed method was validated for linearity, accuracy and robustness.
Results:The proposed method was found to be linear in the range of 6 to 18 µg/ml. The correlation coefficient (r 2
Conclusion:The analytical technique presented here demonstrates shorter and easier sample preparation method, decreased analysis time and reduces the need for complicated or expensive equipment. The sample preparation method used in this study can also be further extended to higherend analytical techniques and other biological samples for quantification of bosentan.) was found to be 0.99. The results revealed that the linearity, accuracy and robustness of the developed method were within the acceptable range.
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