Root-knot nematodes (RKN), Meloidogyne sp. hinders functioning of crops and causes global losses in terms of productivity and yield. Meloidogyne sp. are microscopic, obligatory endoparasites with ubiquitous distribution in different parts of the world. Taking into consideration these aspects, the present study was conducted to explore nematicidal activity of the Streptomyces hydrogenans strain DH-16 against M. incognita to regulate its pathogenicity in plants. In-vitro experimentation revealed that pretreated seeds with solvent and culture supernatant lowered root galls in infested plants and promoted growth of Solanum lycopersicum seedlings, revealed through the morphological analysis. Additionally, antioxidative defense responses were induced with microbes. However, oxidative stress markers were considerably reduced after microbial inoculations. Apart from this, secondary metabolites were assessed and modulated in RKN infested plants on microbial supplementations. Confocal studies evaluated glutathione accumulation within root apices and its enhancement was directly proportional to defense responses. Therefore, the current study concluded the role of S. hydrogenans in stimulating antioxidant potential against RKN along with growth promoting aids. Thus, the outcome of the current study endorses that metabolites produced by S. hydrogenans can be used as safe biocontrol agents against M. incognita and also as plant growth promoting agents.

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Plants, being sessile organisms, are challenged by variety of stresses in the form of abiotic and biotic components of environment. In such adverse conditions, plants evolve various adaptive strategies in order to survive. Among these strategies, accumulation of polyamines in plants helps to combat stress. Polyamines are ubiquitous low molecular weight compounds, which are now known to be involved in stress tolerance as suggested by various pieces of evidence during abiotic and biotic stress. The anti-stress properties of polyamines in plants are illustrated by their roles in modulation of morphological growth parameters, elevation of photosynthetic pigments, as well as declined content of stress indices, antioxidative enzymes, and non-enzymatic antioxidants content. The emerging role of polyamines in plant stress tolerance has been discussed in the present review.

Hydrogen sulfide (H2S) is predominantly considered as a gaseous transmitter or signaling molecule in plants. It has been known as a crucial player during various plant cellular and physiological processes and has been gaining unprecedented attention from researchers since decades. They regulate growth and plethora of plant developmental processes such as germination, senescence, defense, and maturation in plants. Owing to its gaseous state, they are effectively diffused towards different parts of the cell to counterbalance the antioxidant pools as well as providing sulfur to cells. H2S participates actively during abiotic stresses and enhances plant tolerance towards adverse conditions by regulation of the antioxidative defense system, oxidative stress signaling, metal transport, Na+/K+ homeostasis, etc. They also maintain H2S-Cys-cycle during abiotic stressed conditions followed by post-translational modifications of cysteine residues. Besides their role during abiotic stresses, crosstalk of H2S with other biomolecules such as NO and phytohormones (abscisic acid, salicylic acid, melatonin, ethylene, etc.) have also been explored in plant signaling. These processes also mediate protein post-translational modifications of cysteine residues. We have mainly highlighted all these biological functions along with proposing novel relevant issues that are required to be addressed further in the near future. Moreover, we have also proposed the possible mechanisms of H2S actions in mediating redox-dependent mechanisms in plant physiology.

The current study assessed the nematicidal and plant growth promoting potential of metabolites produced by Streptomyces hydrogenans strain DH-16 on morphological and physiological activities in 60 days old Solanum lycopersicum plants grown under Meloidogyne incognita stress. M. incognita infestation altered the levels of various photosynthetic pigments, various stress markers, enzymatic and non-enzymatic antioxidants in S. lycopersicum plants grown under in-vivo conditions. However, treatment with culture cells, supernatant and extract produced by S. hydrogenans strain DH-16 significantly reduced the number of galls in M. incognita infested plants when compared with untreated M. incognita infected plants. Moreover, the culture cells/ supernatant/ extract remarkably lowered the levels of stress markers (Hydrogen peroxide and Malondialdehyde) in infected plants and enhanced the activities of non-enzymatic antioxidants (glutathione, tocopherol) and enzymatic antioxidants (Catalase, Superoxide dismutase, Ascorbate peroxidase, Guaiacol peroxidase, Gluatathione-S-transferase and Polyphenol oxidase) in metabolites treated M. incognita infected plants. The enhanced level of different photosynthetic attributes were also evaluated by studying gas exchange parameters and different plant pigments. Moreover, an increment in the content of phenolic compounds such as total phenols, anthocyanin and flavonoids were also reflected in treated and nematode infested plants. The present study also evaluated the microscopic analysis depicting cell viability, nuclear damage and hydrogen peroxide localization in differently treated plants. The outcome of the present study therefore endorses the efficacy of DH-16 as a potential biocontrol agent that help plants in mitigating M. incognita stress.

The agricultural sector is a foremost contributing factor in supplying food at the global scale. There are plethora of biotic as well as abiotic stressors that act as major constraints for the agricultural sector in terms of global food demand, quality, and security. Stresses affect rhizosphere and their communities, root growth, plant health, and productivity. They also alter numerous plant physiological and metabolic processes. Moreover, they impact transcriptomic and metabolomic changes, causing alteration in root exudates and affecting microbial communities. Since the evolution of hazardous pesticides and fertilizers, productivity has experienced elevation but at the cost of impeding soil fertility thereby causing environmental pollution. Therefore, it is crucial to develop sustainable and safe means for crop production. The emergence of various pieces of evidence depicting the alterations and abundance of microbes under stressed conditions proved to be beneficial and outstanding for maintaining plant legacy and stimulating their survival. Beneficial microbes offer a great potential for plant growth during stresses in an economical manner. Moreover, they promote plant growth with regulating phytohormones, nutrient acquisition, siderophore synthesis, and induce antioxidant system. Besides, acquired or induced systemic resistance also counteracts biotic stresses. The phytomicrobiome exploration is crucial to determine the growth-promoting traits, colonization, and protection of plants from adversities caused by stresses. Further, the intercommunications among rhizosphere through a direct/indirect manner facilitate growth and form complex network. The phytomicrobiome communications are essential for promoting sustainable agriculture where microbes act as ecological engineers for environment. In this review, we have reviewed our building knowledge about the role of microbes in plant defense and stress-mediated alterations within the phytomicrobiomes. We have depicted the defense biome concept that infers the design of phytomicrobiome communities and their fundamental knowledge about plant-microbe interactions for developing plant probiotics.

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