Foxtail millet (Setaria italica) is a cultivated nutritional cereal, which originated in South Asia and is considered one of the oldest cultivated millets in India. DNA fingerprinting is mandatory for registration of newly developed varieties with National Bureau of Plant Genetic Resources (NBPGR) and Protection of Plant Varieties and Farmers' Rights Authority (PPV&FRA). Due to the limited availability of genomic information in foxtail millet, the use of DNA based markers in fingerprinting of crop varieties is also limited. Hence in the present investigation, available RAPD and SSR markers of cereals are used for fingerprinting the foxtail millet varieties. The newly released variety ATL 1 is differentiated from popular variety CO (Te) 7 using SSR and RAPD markers. About 66 maize SSR primers, 16 sorghum SSR primers, and 10 RAPD primers were used in the study. Out of 66 maize SSR markers used for study, one showed polymorphism. The marker umc1704 showed polymorphism between CO (Te) 7 and ATL 1 by the presence of 670 bp allele CO (Te) 7. The RAPD primers OPB4, OPA5, OPA11 and OPB1 also helped for differentiation of the two varieties. The identified makers will help for genetic purity testing of CO (Te) 7 and ATL 1 in the seed chain.
Background: Earthworms are the most important soil invertebrate and have been used for exploring potent therapeutic agents. Perionyx excavatus (Perrier, 1872) is a tropical earthworm species widely used in vermicomposting worldwide, which has a prodigious regeneration capability. Objectives: This study was carried out to isolate fluorescence-emitting microbiota from the gut of earthworm, P. excavatus, and characterize the fluorophores along with finding the symbiotic association in regenerating worms. Methods: Gram staining was performed to selectively identify the fluorescence-emitting bacterial colonies and further confirmed using 16S ribosomal RNA (rRNA) sequencing. Thin layer chromatography (TLC), ultraviolet-visible spectroscopy (UV-Vis), and Fourier-transform infrared spectroscopy (FTIR) were carried out to separate and characterize the isolated fluorophores. Further, fluorescence microscopy was used to examine the autofluorescence property. Results: Gram staining results showed that the isolated fluorescence-emitting gut bacterium is Gram-positive and it is rod-shaped. Molecular characterization confirmed that the isolated fluorescence-emitting bacterium is Priestia filamentosa. Moreover, two fluorophores responsible for emitting fluorescence, known as unknown fluorescent compound 1 (CF1), and fluorescent compound 2 (CF2) were separated and partially characterized. Anti-inflammatory assays confirm that the fluorophores have very good anti-inflammatory properties. In addition, these fluorophores were expressed in the epithelial cell layer of earthworm, P. excavatus, during regeneration. Notably, bright, intense fluorescence was observed high in regenerating worms compared to control worms. Conclusions: Hence, these two fluorophores will be studied in-depth in the context of structural conformation, chemical composition, and functional characteristics for potential biotechnological applications.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.