DNA fingerprinting of foxtail millet (Setaria italica L.) variety ATL 1 using SSR and RAPD markers along with morphological descriptors

Natesan, Senthil Kumar; Murugesan, Gowtham Krishnan; Murugan, Nandhini; Chandran, Sarankumar; Nirmalakumari, A.

doi:10.22271/tpr.2020.v7.i3.072

Cited by 3 publications

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“…Four different cytoplasmic groups among the populations from cytoplasmic genetic sequences were identified, which have to be further increased in the near future to overcome cytoplasmic-specific pathogens ( Liu et al, 2019a ). Further, fingerprinting foxtail millet varieties using SSR and RAPD markers helped assess the genetic purity between CO (Te) 7 and the newly released variety, ATL1 ( Natesan et al, 2020 ).…”

Section: Omics Resources In Setariamentioning

confidence: 99%

Multi-omics intervention in Setaria to dissect climate-resilient traits: Progress and prospects

et al. 2022

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Millets constitute a significant proportion of underutilized grasses and are well known for their climate resilience as well as excellent nutritional profiles. Among millets, foxtail millet (Setaria italica) and its wild relative green foxtail (S. viridis) are collectively regarded as models for studying broad-spectrum traits, including abiotic stress tolerance, C4 photosynthesis, biofuel, and nutritional traits. Since the genome sequence release, the crop has seen an exponential increase in omics studies to dissect agronomic, nutritional, biofuel, and climate-resilience traits. These studies have provided first-hand information on the structure, organization, evolution, and expression of several genes; however, knowledge of the precise roles of such genes and their products remains elusive. Several open-access databases have also been instituted to enable advanced scientific research on these important crops. In this context, the current review enumerates the contemporary trend of research on understanding the climate resilience and other essential traits in Setaria, the knowledge gap, and how the information could be translated for the crop improvement of related millets, biofuel crops, and cereals. Also, the review provides a roadmap for studying other underutilized crop species using Setaria as a model.

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Section: Omics Resources In Setariamentioning

confidence: 99%

Multi-omics intervention in Setaria to dissect climate-resilient traits: Progress and prospects

et al. 2022

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“…Moreover, their application demands a minute quantity of DNA for polymerase chain reaction (PCR) analysis, facilitating the detection of polymorphisms [38,. SSRs emerge as invaluable resources for evaluating genetic diversity, identifying potential allelic sources for the augmentation of key traits, and delving into the genomic evolution and historical trajectory of cultivars [39][40]. Numerous studies have spotlighted the utility of DNA markers in millet cultivar identification, exemplified by Avadh et al [41], Kumar et al [42], and Nagawade et al [43].…”

Section: Introductionmentioning

confidence: 99%

“…Numerous studies have spotlighted the utility of DNA markers in millet cultivar identification, exemplified by Avadh et al [41], Kumar et al [42], and Nagawade et al [43]. Molecular markers have been harnessed across diverse crops to establish DNA fingerprints [44], facilitate seed purity assessments, underpin variety registration systems, support Distinctness, Uniformity, and Stability (DUS) examinations [40], aid in variety protection [39], and enable hybridity testing [45]. The present investigation conducted with aim to assess the purity of true hybrids, and their parental lines using SSR molecular markers.…”

Section: Introductionmentioning

confidence: 99%

Detection of True Hybrids in Pearl Millet Cross Combinations by Employing SSR Molecular Markers

Patel,

Tripathi,

Shrivastava

et al. 2023

IJECC

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Pearl millet (Pennisetum glaucum L. R. Br.), a crucial staple food and significant cereal crop, is gaining prominence due to its versatile applications as feed, food, and fodder. Heterosis of this crop has been extensively harnessed to increase productivity. Hybrid variants exhibit superior grain and stover yields compared to open-pollinated varieties.Top of Form The primary aim of this investigation was to evaluate and confirm authentic hybrids within three resultant F1 progenies. The assessment of parents and F1 hybrids was carried out during the Kharif 2021for the purpose of accurate discrimination and rapid verification of true hybrids by employing 20 SSR molecular markers. The experimental materials consisted of three distinct cytoplasmic male sterile (CMS) lines including ICMA 843-22, ICMA 04999, and ICMA 02333, used as female parents along with three fertility restorers, viz., ICMR 01004, ICMR 20233, and ICMR 20342, were utilized as male parents. The analysis of SSR profiles was based on distinctive banding patterns, resulting in unique profiles for the hybrids. The amplified fragment sizes ranged between 90 to 300 base pairs (bp), effectively enabling the differentiation of authentic hybrids. Within the specific crosses, the percentage of polymorphism was determined 75% for the cross ICMA 843-22 × ICMR 01004, 80% for the cross combination ICMA 04999 × ICMR 20233, and 75% for the cross ICMA 02333 × ICMR 20342. The true hybrids were calculated using hybrid purity percentage formula using heterozygous banding patten among total plants evaluated. Among a total of 100 F1 plants, 85, 86, and 88 plants were accurately identified as true hybrids in the respective crosses i.e., ICMA 843-22 × ICMR 01004, ICMA 04999 × ICMR 20233, and ICMA 02333 × ICMR 20342. The identified markers hold significant potential for applications such as hybridity test, genetic purity assessments, diverse germplasm identification, and DNA fingerprinting endeavors in future.

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An Evaluation Model of Plant Variety Rights Capitalization Operation Value Based on Big Data Analysis

et al. 2022

Scientific Programming

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Plant variety rights are essential for agricultural development and food security. This paper mainly starts with the cost-benefit issue of plant variety rights that enterprises are concerned about, and introduces the cost-benefit analysis method into the research and development, application, and protection of plant variety rights. This research takes the new plant variety rights as the research object, clarifies the development status of the world plant variety rights from the perspective of time series development characteristics and spatial distribution pattern, explores the location of the variety rights in the world, and uses methods such as knowledge measurement and data mining, to carry out in-depth research on the application and authorization of plant variety rights, the scope of protection catalogues, the structure of variety rights, and the applicant unit. Finally, the regression model is tested from five aspects: regression model significance test, model goodness-of-fit test, Hosmer goodness-of-fit test, goodness-of-fit test, and overall accuracy test of model prediction. The regression coefficient of plant variety rights cooperation in the research process was 1.908, and the statistical test value was significant at the 5% level ( P = 0.022 < 0.05 ). Using system dynamics to evaluate its risks, it provides new ideas for the risk analysis and prevention of seed industry enterprises’ variety rights capitalization operation.

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DNA fingerprinting of foxtail millet (Setaria italica L.) variety ATL 1 using SSR and RAPD markers along with morphological descriptors

Cited by 3 publications

References 0 publications

Multi-omics intervention in Setaria to dissect climate-resilient traits: Progress and prospects

Multi-omics intervention in Setaria to dissect climate-resilient traits: Progress and prospects

Detection of True Hybrids in Pearl Millet Cross Combinations by Employing SSR Molecular Markers

An Evaluation Model of Plant Variety Rights Capitalization Operation Value Based on Big Data Analysis

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