Salmonella enterica is an important foodborne pathogen. Its rapid and reliable detection in food is critical for the effective prevention of the outbreak of foodborne diseases. Here, we report a novel method for the rapid, specific, label‐free, and real‐time detection of S. enterica with biolayer interferometry (BLI) using an antibody as the receptor. The limit of detection for S. enterica in buffer was 1.6 × 105 CFU/ml at a binding time of 300 s. This method enabled the successful identification of S. enterica in complex food matrices (whole egg powder and beef samples) with satisfactory recovery rates. This BLI‐based method holds great promise in serving as a novel diagnostic tool in the field of food safety and other related fields for the rapid, label‐free, specific, and real‐time detection of S. enterica.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.