Rapid label‐free detection of Salmonella enterica with biolayer interferometry

Abstract

Salmonella enterica is an important foodborne pathogen. Its rapid and reliable detection in food is critical for the effective prevention of the outbreak of foodborne diseases. Here, we report a novel method for the rapid, specific, label‐free, and real‐time detection of S. enterica with biolayer interferometry (BLI) using an antibody as the receptor. The limit of detection for S. enterica in buffer was 1.6 × 105 CFU/ml at a binding time of 300 s. This method enabled the successful identification of S. enterica in complex food matrices (whole egg powder and beef samples) with satisfactory recovery rates. This BLI‐based method holds great promise in serving as a novel diagnostic tool in the field of food safety and other related fields for the rapid, label‐free, specific, and real‐time detection of S. enterica.

Phage-layer interferometry: a companion diagnostic for phage therapy and a bacterial testing platform

“…For example, BLI assays have been developed to detect analytes in blood, light soy sauce, and even milk 38 – 42 . In addition, antibodies or phage components, such as receptor binding proteins or lysins, have been used to functionalize BLI biosensors for detecting foodborne pathogens 40 , 41 . Biosensors functionalized with antibodies specific for Salmonella enterica can detect contamination as low as 1.6 × 10 5 colony-forming units per milliliter (CFU/mL) in less than 300 seconds 41 .…”

“…In addition, antibodies or phage components, such as receptor binding proteins or lysins, have been used to functionalize BLI biosensors for detecting foodborne pathogens 40 , 41 . Biosensors functionalized with antibodies specific for Salmonella enterica can detect contamination as low as 1.6 × 10 5 colony-forming units per milliliter (CFU/mL) in less than 300 seconds 41 . Additionally, biosensors functionalized with phage lysin protein have improved limits of detection, being able to detect 13 CFU/mL of Staphylococcus aureus in complex media, such as light soy sauce and ice cubes 40 .…”

Facial Preparation of Cyclometalated Iridium (III) Nanowires as Highly Efficient Electrochemiluminescence Luminophores for Biosensing

“…Table S1: Detection of Salmonella enteritidis by Different Methods. References [ 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 ] are cited in Supplementary Materials.…”