The present study describes phytoassisted synthesis of magnesium oxide nanoparticles with an aqueous extract of Swertia chirayaita. The stable magnesium oxide nanoparticles (MgONPs) formed by this method were spherical particles that were <20 nm in size. The method is cost-effective and eco-friendly. MgONPs from phytoassisted synthesis were characterized by UV-visible (UV-vis) spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD). The UV-vis absorbance spectrum of the synthesized MgONPs displayed a surface plasmon resonance at 510 nm. XRD analysis showed that the MgONPs were crystalline in nature with a face-centred cubic geometry. Further, the MgONPs tested against Gram-positive [Staphylococcus aureus-MTCC-9442, Staphylococcus epidermidis-MTCC-2639, Bacillus cereus-MTCC-9017] and Gram-negative bacteria [Escherichia coli-MTCC-9721, Proteus vulgaris-MTCC-7299, Klebsiella pneumonia-MTCC-9751] by agar-well diffusion method were found to be effective against both Gram-negative bacteria and Gram-positive bacteria.
The ever-increasing use of pesticides in the agricultural and public health has become a major cause of sterility in human and various other animals particularly in males. This study was sought to screen the toxic impacts of cypermethrin (synthetic pyrethroid) on reproduction and development. Twenty-four Wistar male rats divided into four groups were orally administered cypermethrin of daily doses 50, 75, or 100 mg kg À1 bwt per day for 45 days; and for developmental toxicity, 12 female rats were separated into two groups. Maternal rats (experimental) were administered cypermethrin (100 mg kg À1 ) by gavage daily from 6th to 17th day of gestation, and the control group was dosed only vehicle (olive oil). The body weights, fertility index, biochemical, enzymatic, hormonal, and histopathological parameters were the criteria used to evaluate the toxicity of cypermethrin. Study showed significant decline in the weight of testes, epididymises, seminal vesicles, and ventral prostate, and reduction in sperm counts both in epididymises and testes in chemical-treated animals. Pre-and postfertility test showed 50%, 80%, and 100% negative results after treatment. A significant degenerative reduction in testicular glycogen and sialic acid was also noted. In contrast, protein and cholesterol levels of testes were significantly increased. In addition, acid phosphatase activity was significantly increased, while alkaline phosphatase, testosterone, leutinizing hormone (LH), and follicle stimulating hormone (FSH) levels were diminished. Histology of testes showed degenerative changes in seminiferous tubules. Cypermethrin exposure during gestation produced adverse effects markedly in females and fetuses.
The cyanobacterium Spirulina platensis is an attractive source of the biopigment, which is used as a natural colour in food, cosmetic, pharmaceutical products and have tremendous applications in nutraceuticals, therapeutics and biotechnological research. The present study examines the possibility of increasing the content of Phycocyanin, Allophycocyanin, Phycoerythrin and Carotenoids under stress conditions including different pH, salinity and carbon content in S. platensis isolated from Jalmahal, Jaipur (Rajasthan). The production of Phycocyanin, Allophycocyanin and Phycoerythrin were enhanced with 0.4 M NaCl, pH 7 and Carbon deficiency as compared to standard.
The present study explores biological synthesis of silver nanoparticles (AgNPs) using the cell-free extract ofSpirulina platensis. Biosynthesised AgNPs were characterised by UV-Vis spectroscopy, SEM, TEM, and FTIR analysis and finally evaluated for antibacterial activity. Extracellular synthesis using aqueous extract ofS. platensisshowed the formation of well scattered, highly stable, spherical AgNPs with an average size of 30–50 nm. The size and morphology of the nanoparticles were confirmed by SEM and TEM analysis. FTIR and UV-Vis spectra showed that biomolecules, proteins and peptides, are mainly responsible for the formation and stabilisation of AgNPs. Furthermore, the synthesised nanoparticles exhibited high antibacterial activity against pathogenic Gram-negative, that is,Escherichia coli, MTCC-9721;Proteus vulgaris, MTCC-7299;Klebsiella pneumoniae, MTCC-9751, and Gram-positive, that is,Staphylococcus aureus, MTCC-9542;S. epidermidis, MTCC-2639;Bacillus cereus, MTCC-9017, bacteria. The AgNPs had shown maximum zone of inhibition (ZOI) that is31.3±1.11inP. vulgaris. Use of such a microalgal system provides a simple, cost-effective alternative template for the biosynthesis of nanomaterials of silver in a large scale that could be of great use in biomedical applications.
Two Indian spices, Trachyspermum ammi and Myristica fragrans, were studied for their essential oil (EO) yielding pattern, insecticidal activity, antibacterial activity, and composition. The essential oils (EOs) of T. ammi (1.94 ± 30 mL/100 gm) and M. fragrans (5.93 ± 90 mL/100 gm) were extracted using hydrodistillation method. In Gas Chromatography analysis, the beta-pinene, alpha-pinene, alpha-p-menth-1-en-4-ol, Limonene, and elemicin were found as major constituents of T. ammi essential oil whereas M. fragrans essential oil mostly contains Gamma-Terpinolene, p-Cymene, Thymol, and beta-pinene. The insecticidal activities of EO were demonstrated using LC50 values against Plodia interpunctella and EO of T. ammi was found comparatively more effective than EO of M. fragrans. Further, individual EO and combination of essential oil were examined for antibacterial activity against three Gram (−) bacterial strains (E. coli-MTCC 443, P. vulgaris-MTCC 1771, and K. pneumoniae-MTCC number 7028) and three Gram (+) bacterial strains (S. aureus-MTCC 3381, B. subtilis-MTCC 10619, and B. megaterium-MTCC 2412) by well agar diffusion method. The essential oil in combination (CEO) exhibited higher antibacterial activity as compared with individual essential oils.
In the present study, rapid reduction and stabilization of Ag+ ions with different NaOH molar concentration (0.5 mM, 1.0 mM and 1.5 mM) has been carried out in the aqueous solution of silver nitrate by the bio waste peel extract of P.granatum. Generally, chemical methods used for the synthesis of AgNPs are quite toxic, flammable and have adverse effect in medical application but green synthesis is a better option due to eco-friendliness, non-toxicity and safe for human. Stable AgNPs were synthesized by treating 90 mL aqueous solution of 2 mM AgNO3 with the 5 mL plant peels extract (0.4% w/v) at different NaOH concentration (5 mL). The synthesized AgNPs were characterized by UV-Vis spectroscopy, TEM and SEM. Further, antimicrobial activities of AgNPs were performed on Gram positive i.e. Staphylococcus aureus, Bacillus subtilius and Gram negative i.e. E. coli, Pseudomonas aeruginosa bacteria. The AgNPs synthesized at 1.5 mM NaOH concentration had shown maximum zone of inhibition (ZOI) i.e. 49 ± 0.64 in E. coli, whereas Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilius had shown 40 ± 0.29 mm, 28 ± 0.13 and 42 ± 0.49 mm ZOI respectively. The MIC value of 30 μg/mL observed for E. coli Whereas, Staphylococcus aureus, Bacillus subtilius and Pseudomonas aeruginosa had shown 45 μg/mL, 38 μg/mL, 35 μg/mL respectively. The study revealed that AgNPs had shown significant antimicrobial activity as compared to Streptomycin.
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