The experiment was perfumed according to (R.C.B.D) in triplet in field belongs to the department of crop sciences, college of Agricultre, university of Diyala, whose soil has a texture of Slity loam during spring season of 2019, to study the effect of addition of three different types of fertilizers to the soil which are Humic acid 300 kg.h-1, Trichoderma 75 kg.h-1 and NPK 200 Kg.h-1 , on the growth and characters of Panicum Maximum cv Mombasa. Results showed significant superiority of humic acid fertilizer after 40 days of fertilization with regards to the plant height, branches number, leaves number, leaf area, the total yield of green feed and hay yield. In fertilization treatment with tricoderma, the weed after 40 days was outstanding in the characters of the plant height, branches number, leaves number, leaf area, the total yield of green feed and hay yield, treatment with NPK, the cut after 40 days exceeded in the plant height , branches number, leaves number, leaf area , the total yield of green feed and the hay yield.
The highest specific activity for (C12O) enzyme purification was within the second stage of the purification steps represented by precipitation with ammonium sulfate AS 2% (weight / volume), reaching about 14.56 U. mg -1 after achieving the purification by 6.6 folds with a recovery rate of 78.3%. The optimum catalytic activity of the enzyme (C12O) was within the pH of 6.5-8.5 and the temperature of 25 ° C. Immobilization of (C12O) enzyme by biosilica of diatom frustules DFS/G GA gave a double shifting of 20 ° C from the optimum temperature for enzyme activity, within all the purification stages, as the percentage of catechol degradation through it at the temperature of 45 ° C reached about 93% and 87.2% and 85% were in the precipitation stage with ammonium sulfate AS and the precipitation with streptomycin sulfate SS and the enzymatic crude extract CEE respectively. While the shifting was only 10 ° C in the case of immobilization on polymeric supports types: PAN / G and PAA / G. In contrast, the soluble (C12O) enzyme which did not show any shifting by its optimum temperature , and its highest activity values were within the optimum temperature of 25 ° C. While the method of immobilization for (C12O) enzyme by biosilica of diatom frustules DFS/G gave a 1.5-degree shifting for the optimum pH of the enzyme activity, within all the purification stages, the highest rates of catechol degradation were reached through it at the base pH 9-10 about 91.8% and 86.5. % And 85.0% in the AS, CEE and SS stages, respectively. Whereas, the enzyme immobilization methods of (C12O) by PAN / G and PAA / G did not show such displacement during the enzymatic purification stage with streptomycin sulfate SS but were limited to the primarily stages of enzymatic purification. , As it was able to shift the optimal pH of the (C12O) enzyme by 1.5 degrees also within the purification stages of AS and CEE. The rates of catechol biodegradation through it at the base pH 9-10 were about 79.0% and 71.8%, respectively, in the case of immobilization by PAN /G and PAA / G with percentages of 71.8% and 62.0%, respectively. In contrast, soluble (C12O) enzyme did not show any shifting by its optimum pH, and its highest activity values were within the optimum pH of 6.5-8.5. The reason for these results may be attributed to the role of the large surface area ready for covalent immobilization of (C12O) enzyme by biosilica of diatom frustules compared with the surface area of pores of the two types of polymeric membranes used in current study.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.