Objectives: To determine the effect of lemongrass infusion on the growth of Streptococcus mutans at various concentrations. Methods: This study used post test only control group design with total sample is 9 samples, concentration 100%, 50%, 25%, 12,5%, 6,25%, 3,125%, 1,56%, positive control, and negative control with 3 replications. The research uses dilution method by looking at the turbidity which can be marked as MIC, for MBC used pour plate method was determined by looking at the presence or absence of bacterial growth on the plate. Data analysis was performed using the spss application using Kruskal-Wallis test with significance level if the result of p<0,05. Results of Study: The results of the sample group were 100%, 50%, 25%, 12,5%, 6,25%, 3,125%, 1,56% in the MIC test showed turbidity in all samples, then the MBC test showed growth bacteria in all samples. The assessment of the probability test p value of 1,000 (>0,05) indicates that there is no significant difference between research groups. Conclusion: There was no effect of lemongrass stem infusion with various concentrations on the growth of Streptococcus mutans.
Background: The color of teeth in each individual varies greatly in which it is influenced by the color of dentin and enamel. The most frequent tooth discoloration that happened is caused by extrinsic stains like coffee. Coffee consumption habits are increasing along with the level of creativity in serving coffee. Nowadays, coffee with conventional methods like black/Turkish coffee and manual brewing methods such as drip brewing and espresso become popular coffee that are fancied by Indonesian people. Exposure to chromogenic substances and the acidic nature of coffee can cause the formation of pores in enamel and facilitate the deposition of dyes, which cause the color changes. Aim: The aim of this research was to analyze the effect of brewing methods on tooth discoloration. Method: This research was a true experimental study with a pre-post test control group design. Twenty eight post-extracted first premolar teeth samples divided into 4 groups for drip, espresso, black coffee and control by implementing random allocation. All samples were immersed in each coffee brewing group for 3 hours, 6 hours, and 9 hours. The teeth color was measured using a Chromameter based on the CIE L*a*b system. Statistical tests used Kruskal Wallis and Mann-Whitney test. Result: The Kruskal Wallis test showed differences in the color change ∆a * between the four groups (p < 0.05). Mann-Whitney test indicated a significant difference between the treatment group (drip, espresso, and black coffee group) and the control group (p < 0.05). Conclusion: Drip coffee, espresso and black coffee brewing methods can affect tooth discoloration, especially affects the red discoloration. But there is no difference in the effect of the drip, espresso, and black coffee brewing methods on tooth discoloration.
Background: Composite resin is the most commonly used restorative material. One of properties composite resins is surface hardness can change due to exposure of the restorative material to oral fluids such as alcoholic mouthwash. The degradation that occurs due to alcoholic mouthwash is able to reduce the surface hardness of the nanohybrid composite resin. Glycerin is able to increase the hardness of the composite resin by perfecting the polymerization that occurs in the restorative material when exposed to light cured light. Objectives: This study aims to determine the effect of glycerin application on the hardness of nanohybrid composite resins after immersion in alcoholic mouthwash. Methods: A total of 32 samples of nanohobride composite resin in this study were made in the form of a cylinder with a diameter of 5 mm and a thickness of 2 mm. The nanohybrid composite resin samples were divided into control and treatment group by applying glycerin on the sample surface before light curing. The study was continued by immersing the sample into artificial saliva for 24 hours and alcoholic mouthwash for 6 hours. The research continued with surface hardness test using the Vickers Hardness Tester. Result : Paired bivariate T test showed a significant difference between the two groups (p<0.005). The conclusions of this study indicate that the application of glycerin has an effect on the hardness of the nanohybrid composite resin after immersion with alcoholic mouthwash. Conclusion: The application of glycerin affects the hardness of the nanohybrid composite resin after immersion with alcoholic mouthwash. Key words: Glycerin application, Hardness, Nanohybrid composite resin
Introduction: Tooth discoloration is a change in color that occurs on the enamel and dentin surfaces of a tooth due to the habit of drinking tea. Some fruits are used as natural teeth whitening ingredients, such as Manalagi Apple (Malus sylvestris), which contains malic acid. Method: This study was an experimental study with a pre-and post-test control group design involving 27 samples divided into three groups, Control (K), P1, and P2. We immersed tooth samples from three groups in tea solution for two weeks. After that, the P1 group was immersed with 50% Manalagi Apple extract three times a day for one week and the P2 group three times a day for two weeks, while the Control group (K) did not get any treatment. We analyzed the tooth discoloration before and after immersing 50% of Manalagi Apple extracts utilizing a Colorimeter, with hunter L, a, and b color scales. The color difference value was obtained using the formula of ⧍E(Lab) = [(L)2+(a)2+(b)2]1/2]. Results: The ANOVA test results showed significant differences between the three sample groups (p = 0.00). Besides, the Post-hoc test revealed a significant difference between the K group and the P1 group with a p-value of 0.00 (p <0.05). It also indicated a significant difference between the K group and the P2 group with a p-value of 0.00 (p <0.05). Conclusions: Color changes occurred after immersing the teeth in Manalagi Apple extract for one and two weeks. Immersing the teeth for two weeks caused whiter teeth compared to immersing the teeth for one week.
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