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Background:Intestinal parasitic infections (IPIs) are widely distributed throughout the world and have been identified as one of the most significant causes of illnesses and diseases. Accurate diagnosis is essential for proper management and to guide the design, implementation and monitoring of programs for community control of infectious diseases. Objective: To evaluate and compare mini-FLOTAC technique with the widely used formol-ethyl-acetate concentration method (FECM) in IPIs detection. Material and Methods: One hundred fecal samples were randomly collected from different laboratories. All samples were microscopically examined using mini-FLOTAC and FECM. Iodine and modified Zeil Neelsen stains were used to confirm the presence of protozoa cysts, and oocysts of Cryptosporidium spp. and C. cayetanensis, respectively. Results: About two thirds of samples (68%) were positive; 70.6% (48/68) contained only one parasitic infection by at least one method. Generally, the most frequent was Cryptosporidium spp. 35.3% (24/68) followed by E. histolytica/dispar 23.5% (16/68) and G. intestinalis 14.7% (10/68). It was observed that mini-FLOTAC detected the highest number of helminthes infections (100% sensitivity) in contrast to 41.1% for protozoa whereas FECM was the most sensitive approach for protozoa infections (98.2% sensitivity) in contrast to 68.7% for helminthes. Conclusion: Mini-FLOTAC is a safe rapid device for microscopic examination of stool samples with high sensitivity, affordability, and appropriateness of diagnosis in resource-limited settings where IPIs are widespread.The aim of the study is to evaluate and compare the recently developed mini-FLOTAC technique with the widely used FECM in IPIs detection.

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Introduction: Intestinal parasitic infections (IPI) are still public health problems in many communities, particularly among children in developing countries.This crosssectional study aimed to investigate the current prevalence and risk factors associated with intestinal polyparasitism (the concurrent infection with multiple intestinal parasite species) among schoolchildren in Sohag governorate, Egypt. Methodology / Principal findings: Fecal samples were collected from 200 schoolchildren (51.5% boys and 48.5% girls), preserved and examined by using formalin-ethyl acetate concentration technique and modified Kinyoun acid fast stain. Demographic information was collected by using a standardized questionnaire. Overall, 63.5% of the children were found to be positive for at least one parasite species. Of these, 23.5% were polyparasitized. The overall prevalence of Cryptosporidium, Giardia duodenalis, Entamebahistolytica/ dispar, Blastocystisinfections were 34%, 14.5% and 13%, respectively. Univariate and multivariable logistic regression models showed that none of the age, gender, residence or family size were risk factors for polyparasitism. Conclusions/Significance: Intestinal polyparasitism is prevalent among children in Sohag, Egypt. Hence, effective and sustainable control measures, including schoolbased periodic chemotherapy, providing adequate health education focused on good personal hygiene practices and proper sanitation, as well as safe drinking water supply should be implemented to reduce the prevalence and consequences of these infections in this population.

The diagnosis of acute infection is established by the presence of tachyzoites in tissue parts and/or smears of body fluid (CSF, amniotic, or bronchoalveolar lavage fluids) [6] . The presence of several tissue cysts indicates the presence of a chronic infection [7] . In experimentally infected mice, histopathological smear analysis revealed that the chosen tissues for detecting T. gondii are the liver and spleen in the virulent strain (RH), and the brain and kidney in the avirulent strain (ME49) [8] .

Introduction:Intestinal parasitic infections are widely distributed throughout the world and have been identified as one of the most significant causes of illnesses and diseases among the disadvantaged population .This study aimed to evaluate Formol-ethyl acetate concentration method in detection of intestinal parasitic infections. Methodology / Principal Findings: 100fecal samples were collected, preserved and examined by using formol-ethyl acetate concentration method.Formal-ethyl acetate concentration method (FECM) detectedprotozoan infections in55%of the samples while only 8%helminthic infections were detected. Conclusions/Significance:The concentration techniques recommended for the qualitative diagnosis of intestinal parasites (both helminths and intestinal protozoa) are the formol-ethyl acetate concentration method (FECM). By centrifugation, it leads to the recovery of all protozoa, eggs, larvae, coccidian and microsporidia present; This method have the advantages of being rapid and suitable for fresh or preserved stool, also used for concentrating parasites on which Zinc sulfate floatation has given poor results due to excessive amounts of fats and fatty acids, and for operculated ova of some trematodes and cestodes, the morphology of most parasite is retained for easy identification and it covers most intestinal parasites.