Evaluation OfFormol-ethyl acetate Concentration Method In The Diagnosis Of IntestinalParasitic Infections

Abstract

Introduction:Intestinal parasitic infections are widely distributed throughout the world and have been identified as one of the most significant causes of illnesses and diseases among the disadvantaged population .This study aimed to evaluate Formol-ethyl acetate concentration method in detection of intestinal parasitic infections. Methodology / Principal Findings: 100fecal samples were collected, preserved and examined by using formol-ethyl acetate concentration method.Formal-ethyl acetate concentration method (FECM) detectedprotozoan infections in55%of the samples while only 8%helminthic infections were detected. Conclusions/Significance:The concentration techniques recommended for the qualitative diagnosis of intestinal parasites (both helminths and intestinal protozoa) are the formol-ethyl acetate concentration method (FECM). By centrifugation, it leads to the recovery of all protozoa, eggs, larvae, coccidian and microsporidia present; This method have the advantages of being rapid and suitable for fresh or preserved stool, also used for concentrating parasites on which Zinc sulfate floatation has given poor results due to excessive amounts of fats and fatty acids, and for operculated ova of some trematodes and cestodes, the morphology of most parasite is retained for easy identification and it covers most intestinal parasites.