Piper betle L. var Nigra commonly known as black betel have a potential as drug raw materials. The leave of black betel can be used to resist bleeding so as to accelerate the healing of wounds on the skin, sputum, other uses are for epistaxis, as well as for dialysis, asthma, bronchitis, cough, and high blood pressure. Many uses of black betel leaves make it interesting to learn its metabolites compounds by phytochemical screening. So, this research aimed to isolate and identificate secondary metabolites of Piper betle L. var Nigra. This research used three kind of organic solvents, there were methanol, ethyl acetate, and n-hexane. Several screening tests were used to isolate and identificate the phytochemical compound, including screening of alkaloids, terpenoids/steroids, flavonoids, polyphenols, tannins, and saponins. The results showed that methanol extract of black betel leaves contained of alkaloids, terpenoids/steroid, flavonoids, polyphenols and tannins compounds. The ethyl acetate extract of black betel leaves contained of terpenoids/steroid, flavonoids and tannins compounds. The n-hexane extract of black betel leaves contained of terpenoids/steroid. The methanol extract of Piper betle L. var Nigra contained more secondary metabolites than n-hexane and ethyl acetate extracts. Keywords : methanol ; Phytochemical screening ; Piper betle L. var Nigra.
Elephantopus scaber L. is a plant that has potential as traditional medicine. Callus induction and production of secondary metabolite content can be increased by culture callus using plant growth regulators. This study was purposed to investigate the effect of IBA and kinetin concentration on the induction and secondary metabolite profile of callus from E. scaber L. leaves. Leaves explant of E. scaber L. were cultured on MS medium with various combination concentrations of IBA and kinetin for 6 weeks and then callus was extracted using methanol. Secondary metabolite content from the resulting extract was analyzed using the phytochemical screening method. The result showed that the treatment of IBA 2.0 mg/L and kinetin 1.0 mg/L and treatment of IBA 2.0 mg/L and kinetin 2.5 mg/L are the fastest combination concentration to induce callus at 5.33 ± 0.577 days. Treatment of IBA 2.0 mg/L and kinetin 2.5 mg/L produced callus with the highest fresh weight and dry weight at 0.7016 ± 0.0588 grams and 0.0766 ± 0.0062 grams, respectively. The morphology of calluses grown during this study was compact with various colors appearance, such as light green, brownish green, and brown. Secondary metabolite content of methanol extract of callus E. scaber L. are flavonoids, alkaloids, terpenoids, and saponins.
Callus culture is one of the plant tissue culture techniques that used to study aspects of plant nutrition, somatic embryogenesis, cell suspension culture, secondary metabolite production, and genetic transformation. Callus induction was carried by adding growth regulators. Black betel (Piper betle L. var. nigra) is a medicinal plant that has the potential to produce secondary metabolites. The aim of this study was to obtain the best formula for callus induction of P. betle L. var. nigra. The single growth regulator used is 2,4-Dichlorophenoxyacetic acid (2,4-D), Benzyl Amino Purine (BAP), Indole Butyric Acid (IBA), Indole Acetic Acid (IAA), and Naphthalene Acetic Acid (NAA) with variations the concentration used is 0; 0.5; 1; 1.5; 2; 2.5 mg/L. Each treatment consisted of 6 replicates grown using Murashige and Skoog (MS) medium in vitro for eight weeks. The results showed that the 1.5 mg/L 2,4-D treatment produced the highest fresh weight (1389.5 mg) and dry weight (55.7 mg). Callus P. betle L. var. Nigra in various treatments showed various textures such as compact and friable with callus colors such as white, greenish-white, brownish-white, yellowish-green, greenish-yellow, brownish-yellow, greenish-brown, brown, and gray.
Piper betle L. var. Nigra (black betel) contains secondary metabolites and has biological activity as antibacterial, antifungal, antioxidant, etc. To increase the production of secondary metabolites, an alternative method is needed, namely cell suspension culture. This study aims to determine the effect of abiotic and biotic elicitor on callus biomass produced from cell suspension culture. Leaf explants were grown on Murasige and Skoog (MS) medium with the addition of growth regulator 2.4-D 0.5 mg/L and BAP 2.0 mg/L with abiotic elicitors CuSO4, ZnSO4, HgCl2 and CoCl2 with a concentration of 0.5; 1.0 and 2.5 mg/L. The biotic elicitor used was Aspergillus niger with a concentration of 0.025%; 0.050% and 0.1%. The cultures were incubated for 8 weeks. 0.5 g callus was subcultured on 25 mL cell suspension medium. The suspension culture was shaken at 110 rpm. In this suspension culture, the callus was incubated for 3 weeks. After 3 weeks of age callus on suspension medium was harvested and weighed the fresh and dry weight. The results showed that the highest average dry weight was found in the treatment with abiotic elicitor CuSO4 0.5 mg/L at 0.088 g.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.