Callus induction of leaf explant Piper betle L. Var Nigra with combination of plant growth regulators indole-3-acetic acid (IAA), benzyl amino purin (BAP) and kinetin

Junairiah,; Zuraidassanaaz, Nabilah Istighfari; Izdihar, Fairuz Nabil; Manuhara, Yosephine Sri Wulan

doi:10.1063/1.5004305

Cited by 6 publications

(4 citation statements)

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“…The formation of compact callus in the treatments of 1.0 mg/L BAP and 1.0 mg/L NAA, and 2.0 mg/L BAP and 1.0 mg/L NAA might be attributed to the role of cytokinins such as BAP in the transportation of nutrients. The osmotic potential inside cells is influenced by the cytokinin transport system whereas the turgor pressure is influenced by the presence of sucrose in the medium (Junairiah et al, 2017). This ultimately results in the formation of highly rigid cell walls that clump together to form compact callus.…”

Section: Resultsmentioning

confidence: 99%

Callus Induction of Fig (Ficus carica cv. Violette de Soillès) via Thin Cell Layer Technique

Dahmendra Sriskanda,

Xuan Jia Chew,

Bee Lynn Chew

2022

JTPP

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Ficus carica L. is a fruit-bearing fig plant belonging to the Moraceae family. The fig fruit is known for its health benefits with high levels of fibre, vitamins, sugars and minerals. The leaves are also used for diabetes, cholesterol reduction and skin disorders. Previous reports have documented on the different in vitro callus induction methods of F. carica via plant tissue culture. Thin cell layer (TCL) has been previously established for the micropropagation of many plant species, utilising thin sections of explants in this method to induce callus. The current study aimed to evaluate the efficiency of the TCL technique in the callus induction of F. carica cv. Violette de Soillès. Stem explants with a diameter of 3.0 to 4.0 mm were excised into the thickness of approximately 0.5 to 0.8 mm and inoculated on MS media supplemented with BAP and NAA at different concentrations. The results indicated that MS media supplemented with 1.0 mg/L BAP and 0.5 mg/L NAA, and 2.0 mg/L BAP and 0.5 mg/L NAA induced the highest amount of semi-friable callus with 100% callus induction rate. The current study reported on the induction of callus from the Violette de Soillès cultivar via TCL, which in future, can be explored for shoot organogenesis, somatic embryogenesis and cell suspension culture.

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Section: Resultsmentioning

confidence: 99%

Callus Induction of Fig (Ficus carica cv. Violette de Soillès) via Thin Cell Layer Technique

Dahmendra Sriskanda,

Xuan Jia Chew,

Bee Lynn Chew

2022

JTPP

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“…Traditionally, the betel leaves have been considered as herbal remedy. Chewing the raw leaves can stimulate the central nervous system activity and treat several diseases, such as high blood pressure, wounds, asthma, cough and bronchitis (Junairiah et al 2017). The content of saponins and tannins in betel leaves is known as antiseptic for wounds and anti-inflammatory agent (Novita 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies have shown that black betel in Indonesia is rich in phytochemical compounds and has the potential to be developed as supplement to maintain health (Junairiah et al 2017). Suriani et al (2020) stated that the black betel has its own unique volatile oil, commonly known as betel oil.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, Prasetya et al (2021b) mentioned that 2-octenoic acid and 2-hexenoic acid, both fatty acid derivatives, were successfully extracted from the betel leaves. Indonesia's black betel anti-bacterial assays show that there is antimicrobial activity against pathogenic microbes such as Staphylococcus aureus, Escherichia coli, Candida albicans, Streptococcus mutans and Streptococcus sanguinis (Junairiah et al 2017;Prasetya et al 2012). Black betel can also be used as an eco-friendly biocontrol agent in organic rice cultivation.…”

Section: Introductionmentioning

confidence: 99%

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Anatomical, morphological and physiological leaf characters of black betel (Piper betle L. var. nigra) in varying natural and man-made habitats

et al. 2023

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Abstract. Kuswandi PC, Ariyanti NA, Yunus MF, Amri CNAC. 2023. Anatomical, morphological and physiological leaf characters of black betel (Piper betleL. var. nigra) in varying natural and man-made habitats. Biodiversitas 24: 3236-3244. Piper betle L. var. nigra or black betel (known as Sirih hitam in Indonesia) contains valuable secondary metabolites, such as alkaloids, flavonoids, saponins, tannins, phenols, carotenoids, steroids and terpenoids. Black betel leaf extract has been shown to have antimicrobial activity thus there is a prospect to be developed as a promising herbal plant. Nevertheless, little information is available about its development as a medicinal plant. This research studies the influence of different habitats on several characters of black betel leaves with the aim to understand the suitable environmental conditions for the optimum growth of black betel plants. We used a survey method and random sampling of black betel leaves in four locations in Java Island, Indonesia, namely Banyuwangi which represents natural habitat, and Karanganyar, Ngaglik and Pakem which represent man-made habitats. Measurements of temperature, humidity, soil moisture and light intensity were carried out at each location. Analyses of leaf area, leaf water content, total leaf chlorophyll content and flavonoid content were undertaken and statistically analyzed using SPSS software. Leaf transverse sections were also observed. The results showed that the environmental parameters differed in the four locations. Leaves samples from the natural habitat in Banyuwangi were significantly different (P<0.05) from the three man-made habitats for chlorophyll and flavonoid content. For water content, significant difference was only for Banyuwangi samples with those from Karanganyar and Pakem. For leaf area, significant difference was only found between the Banyuwangi samples and Karanganyar. Observations on the transverse cross section of midrib of black betel leaves from the four locations showed structures that are generally found in Piper betle species, namely the presence of an epidermal layer, trichomes in the abaxial part of the leaf, several layers of the hypodermis, visible vascular tissue and the presence of secretion cells. There were several differences in the leaf anatomy such as greater number of trichomes on the leaves from Karanganyar, the secretory cells that were more visible in the leaves from Ngaglik and Banyuwangi and the sclerenchymal tissue that was more visible in the leaves from Banyuwangi. Such differences are likely influenced by variations in environmental parameters thus showing that the man-made habitat in the Karanganyar location can affect leaves characters similar to black betel plant grown in its natural habitat in Banyuwangi.

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Callus Culture as the Method in Providing Antimalarial Compounds of Piper Genus

Suryani

Aneloi

2021

JESSS

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Malaria is still a serious cases all over the world especially in tropical country with a predict number 300-500 millions people infected per year. The resistence cases of these anti-malaria substance make a new effort to find the new strategy against malaria. This study aim to summarize the potential Piper genus as the source of potential antimalarial compound and recent research of callus induction in piper plant to obtain metabolites.The study of Piper genus represent the 11 Piper species were chemically studied and assayed against the plasmodium. The potential antimalarial compound isolates from several Piper genus were 20,60-Dihydroxy-40-methoxydihydro-chalcone,3-Farnesyl-p-hydroxybenzoic acid, Piperine, Chabamide, Benzoic acid derivatives, Guineensine, pellitorine, brachystamide B, sarmentine, and sermentosine, 5,8-Hydroxy-7-methoxyflavone, Prenylated hydroxybenzoic acid, 4-Nerolidylcatechol, Piperitone, Champor, and Viridiflorol (EO). An effort to propagate necessary antiplasmodial resources especially by callus induction has been conducted for 7 Piper species such as Piper betle, P. colubrinumm, P. crocatum, P. longum, P. nigrum, P. permucronatum and P. solmsianum to obtain higher content of secondary metabolites with a different plant growth hormone (PGR) supplementation while there are most of Piper genus have not been well studied. It conclude that callus culture could be the promising method to obtain antimalarial secondary metabolites as antimalarial.

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Callus induction of leaf explant Piper betle L. Var Nigra with combination of plant growth regulators indole-3-acetic acid (IAA), benzyl amino purin (BAP) and kinetin

Cited by 6 publications

References 5 publications

Callus Induction of Fig (Ficus carica cv. Violette de Soillès) via Thin Cell Layer Technique

Callus Induction of Fig (Ficus carica cv. Violette de Soillès) via Thin Cell Layer Technique

Anatomical, morphological and physiological leaf characters of black betel (Piper betle L. var. nigra) in varying natural and man-made habitats

Callus Culture as the Method in Providing Antimalarial Compounds of Piper Genus

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