The connective tissue fibres of various parts of different fascial and aponeurotic planes of the ventral abdominal walls of 26 albino rats were examined with the scanning electron microscope. Five types of collagen fibres could be identified and visualized stereo-scopically: (1) the fine network seen around the muscle fibres; (2) the loose collagenous bundles and their investing reticular network in the deep fasciae; (3) the wavy parallel bundles in the free part of the aponeuroses; (4) the dense mature collagenous bundles and matted collagenous masses in the attached part of the aponeursoes, and (5) the immature fine irregular fibrils seen during healing of wounds. The relationship between these various types and the process of fibrillogenesis of collagenous bundles is discussed. The functional significance of each type is also pointed out.
The surface ultrastructure of tissue-cultured cells derived from the pleural effusion of metastatic scirrhous breast carcinoma in the human MDF-7 cell line was studied by scanning electron microscopy. It was found to form monolayers of various colonies or collections of cells which were either separate or intimately adherent. The surface ultra-structure of the cells was found to be exclusively formed of microvilli. Data suggesting a diagnostic significance of microvilli in cancer cells were discussed. Observations were made which point to the existence of a compensatory absorptive functional activity exerted by the microvilli.
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