The current study aimed to detect specific types of ADAM proteins in the bovine sperm. Histology study of tissue of male reproduction was involved with testis, head, body, and the tail of the epididymis. Results showed that the testis composed of the parenchyma of testis contained the seminiferous tubules which are surrounded by two layers externally tunica vaginalis and internally tunica albuginea. PAS was positive in the seminiferous tubules of testis and lumen, epithelium of the head, and body of epididymis, Whereas Alcian blue was very low intensity stains with sections and contents of the head, body and tail of the epididymis. Fresh ejaculated bovine sperms were collected and separated from seminal plasma, and proteins of sperm were extracted and run on an electrophoresis gel. Then, the bands of proteins have been stained with coomassie stain, after that, the pieces of proteins were cut and separated from gels and amino acids were extracted after the digestion of proteins, and uploaded to a mass spectrometry machine. Our data was profiled and analysed and detected characterised ADAM proteins included: ADAM1, ADAM2, ADAM3A, ADAM10 and ADAM32, and uncharacterized ADAM proteins included: ADAM1B, ADAM7, ADAM9, ADAM12, ADAM15, ADAM17.
The present study has been out to examine macroscopic structure of the primary bronchi and lung in Bee-eaters bird ( Merops orientalis) observed exist within the rib cage, the distal part of the trachea are divided into two primary bronchi (left and right) and the macroscopic appearance of the primary bronchi consists of a short tube extend caudally from syrinx to enter the proximal third of the visceral surface of the lungs through the hilus. The basic unit consisting of the primary bronchi are cartilaginous rings which takes -C -shape. The mean total length of left and right primary bronchi are (1.025 ± 0.15 cm) and (1.075 ± 0.14 cm); and the number of cartilaginous rings in left and right primary bronchi are ( 18.5 ± 0.50) and (18.5 ± 1.50). The lungs are small, pyramidal-shaped, unlobed, bright pink color, and surrounded by thin colorless membrane the pleura and the air sacs. The lung contains two surfaces (dorsal and ventral), two borders ( medial and lateral) and two extremities ( proximal and distal ). The mean total length, width and thickness of the right lung are (1.77 ± 0.17 cm), (0.95 ± 0.15 cm) and (0.4 ± 0.10 cm) while the mean total length, width and thickness of the left lung are (1.6 ± 0.15 cm ), ( 0.9 ± 0.14 cm ) and (0.37 ± 0.02 cm ).
The present study included ten specimens of adult pigeon wood (Columba palumbus) collected from market of Al-Diwanyia city, divided the specimens into two groups five specimens for grossly study and five specimens for histological study. Opened the birds and extracted the right and left bronchi and left and right lungs for the purpose of grossly and histological studies. The study included grossly study for external bronchi. The total length of left bronchi was ( 0.7 ± 0.1 cm ) and right bronchi was ( 0.65 ± 0.05 cm ) and the basic structure of bronchi was the cartilage has – C – shape, also determine relationship bronchi with the neighboring organs. The grossly study involved the lungs right and left, it is found that the lung has two surfaces ( Costal and Visceral ) and two ends ( Distal and Proximal ) also detected relation to organs nearby, found that the average length of the right lung was ( 2.8 ± 0 cm ) while left lung was ( 2.75 ± 0.05 cm ) and average width of right lung was ( 2.45 ± 0.65 cm ) while the left lung was ( 2.7 ± 0.7 cm ). The histological study involves the histological description of external and internal bronchi of the left and right lungs.
Background and Aim: Angiotensin-converting enzyme 2 (ACE2) is expressed and plays functional and physiological roles in different tissues of the body. This study aimed to distinguish the levels of expression of ACE2 in the lung tissue at different ages of rats. Materials and Methods: In this study, 18 male rats were used and divided into three groups according to age. Real-time quantitative polymerase chain reaction (RT-qPCR) was conducted to determine the levels of the quantification of eosinophil cationic protein mRNA transcript. In addition, tissue specimens of the lung were stained with routine hematoxylin and eosin stains. Results: This study confirmed that RT-qPCR amplification plots of ACE2 gene exhibited clearly expression of the lung tissue of rats in the different groups and there are strong different threshold cycles numbers according to the age at 2 weeks, 2 months, and 6-8 months. Consequently, the expression of ACE2 was completely different between groups depending on the age of the rats. The RT-qPCR results showed that the older animal group (age of 6-8 months) had a significantly higher expression of ACE2 than the other animal groups (ages of 2 weeks and 2 months). In the same way, the second group (age of 2 months) had a significantly higher expression of ACE2 than the first group (age of 2 weeks). This study confirmed that the ACE2 expression is influenced by the age of rats. Conclusion: This study concluded that the expression of the ACE2 receptor of coronavirus disease 2019 would be different according to the age of rats, and this result suggested that expression of ACE2 in lung tissue could determine infection and pathogenesis of COVID-19 during different ages of rats or some individual differences.
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