Detection of the ADAM proteins in the ejaculated bovine sperm

Abstract

The current study aimed to detect specific types of ADAM proteins in the bovine sperm. Histology study of tissue of male reproduction was involved with testis, head, body, and the tail of the epididymis. Results showed that the testis composed of the parenchyma of testis contained the seminiferous tubules which are surrounded by two layers externally tunica vaginalis and internally tunica albuginea. PAS was positive in the seminiferous tubules of testis and lumen, epithelium of the head, and body of epididymis, Whereas Alcian blue was very low intensity stains with sections and contents of the head, body and tail of the epididymis. Fresh ejaculated bovine sperms were collected and separated from seminal plasma, and proteins of sperm were extracted and run on an electrophoresis gel. Then, the bands of proteins have been stained with coomassie stain, after that, the pieces of proteins were cut and separated from gels and amino acids were extracted after the digestion of proteins, and uploaded to a mass spectrometry machine. Our data was profiled and analysed and detected characterised ADAM proteins included: ADAM1, ADAM2, ADAM3A, ADAM10 and ADAM32, and uncharacterized ADAM proteins included: ADAM1B, ADAM7, ADAM9, ADAM12, ADAM15, ADAM17.