Objective:The study was done to evaluate the antibacterial effects of three fluoride releasing restorative materials, namely seventh generation dentin bonding agent (Futurabond ® DC, Voco, Germany), resin modified glass ionomer cement (GC Fuji II™ LC, GC Corporation, Tokyo) and Nanoionomer (Ketac™ N100) against Lactobacillus acidophilus [Microbial Type Culture Collection, 10307] and Streptococcus mutans [Microbial Type Culture Collection, 497]. Materials and Methods:The antibacterial effect was analysed using agar diffusion test. The experimental groups were three fluoride releasing restorative materials and control against Lactobacillus acidophilus (MTCC 10307) (Group A) and Streptococcus mutans (MTCC 497) (Group B). The wells in petri dishes were filled with chlorhexidine, resin modified glass ionomer, nanoionomer, bonding agent and were dropped with micropipettes in paper disks, blown dry and light cured. The culture plates were incubated for 24h at 37°c. The antibacterial effect was checked after 24h, 48h and 7days in triplicates.. Results and Conclusion:The results were collected, and statistically analyzed using the ANOVA test to determine the difference between the mean diameters of the inhibition zone observed. All the three restorative materials showed antibacterial activity against Streptococcus mutans and Lactobacillus acidophilus. The antibacterial effect for resin modified glass ionomer and nanoionomer decreased over a period of 7 days whereas for dentin bonding agent there was an increase in the antibacterial effect seen from 24 hours to 48 hours.
Introduction:The study compared accuracy of J Morita-Root ZX mini (multiple frequency based), Woodpecker gold V (multiple frequency based), Coltene Canal pro (multiple frequency based) apex locators with the irrigating solutions, NaOCl, Endosolv, Citric acid, HEBP. Methodology:The actual root canal length of teeth were measured using a 10 size K file under a dental operating microscope. The samples were mounted in alginate model and the electronic working length was recorded in both dry and wet (with irrigants).Variation in the readings of EALs after blockage was compared with actual length (measurement under microscope). The error was subjected to statistical analysis (ANOVA test and Post hoc Bonferroni test). Results:The mean overall (dry and wet canal) error for Root zx mini was 0.116 mm with maximum and minimum error range of:-2.1 to 1.5mm. The overall (dry and wet canal) mean error for Woodpex 5 was 0.305 within a range of: -1 mm to 1.80mm.The overall (dry and wet canal) mean error for Coltene canal pro was 0.271 within a range of : -2.100 to 1.80. Conclusion:Root ZX mini was the most accurate EAL amongst all the three EALs used in this study in both wet and dry conditions. Amongst the 4 irrigants used, all the three EALs recorded the highest deviation from actual length in presence of Citric acid.
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