Antibacterial Efficacy of Three Fluoride-Releasing Restorative Materials Against Lactobacillus acidophilus and Streptococcus mutans: An In vitro Analysis

Abstract

Objective:The study was done to evaluate the antibacterial effects of three fluoride releasing restorative materials, namely seventh generation dentin bonding agent (Futurabond ® DC, Voco, Germany), resin modified glass ionomer cement (GC Fuji II™ LC, GC Corporation, Tokyo) and Nanoionomer (Ketac™ N100) against Lactobacillus acidophilus [Microbial Type Culture Collection, 10307] and Streptococcus mutans [Microbial Type Culture Collection, 497]. Materials and Methods:The antibacterial effect was analysed using agar diffusion test. The experimental groups were three fluoride releasing restorative materials and control against Lactobacillus acidophilus (MTCC 10307) (Group A) and Streptococcus mutans (MTCC 497) (Group B). The wells in petri dishes were filled with chlorhexidine, resin modified glass ionomer, nanoionomer, bonding agent and were dropped with micropipettes in paper disks, blown dry and light cured. The culture plates were incubated for 24h at 37°c. The antibacterial effect was checked after 24h, 48h and 7days in triplicates.. Results and Conclusion:The results were collected, and statistically analyzed using the ANOVA test to determine the difference between the mean diameters of the inhibition zone observed. All the three restorative materials showed antibacterial activity against Streptococcus mutans and Lactobacillus acidophilus. The antibacterial effect for resin modified glass ionomer and nanoionomer decreased over a period of 7 days whereas for dentin bonding agent there was an increase in the antibacterial effect seen from 24 hours to 48 hours.