The localization and density of toluidine blue (TB) stained mast cells (MCs) in lamina propria mucosae and muscular tunic of pig's urinary bladder in order to elucidate their role in organ function, were studied. It was established that in propria, the MCs were localized predominantly close to vessels of microcirculatory bed. Their number was highest in trigonum -4.00±0.84 and corpus -3.44±0.51 (P˂0.001), followed by apex -2.56±0.51 and collum vesicae -1.17±0.38 (P˂0.001). In the muscular tunic, the MCs were localized near the capillaries' basal lamina, in the adventitia of arteries and veins and between the muscle bundles. The highest MCs density was detected in the muscular tunic of collum (16.50±0.51) -and trigonum -16.61±0.50 (P˂0.001), followed by apex -15.89±0.83, and corpus -12.33±0.48 (P˂0.001) vesicae. In conclusion the higher number of MCs in muscular tunic than propria (P˂0.001) allowed to suggest their significant role in the regulation of smooth muscle contractility.
The aim of this study was to investigate the variations as well as the length of A. cystica and its branches in pigs using corrosion casting method with the self-polymerising resin Duracryl® Plus. The method included several steps: hepatectomy, precasting treatment, injection of Duracryl, polymerisation of casting medium, corrosive treatment, cleaning of the corrosion casts, air-drying and preservation of casts. The livers were collected from 12 male 6-month-old pigs (crossbred Landrace×Danube White). With regards to the beginning of A. cystica, 4 variations were observed and grouped as follows: variation A A. cystica detached from R. dexter medialis, together with R. quadratus (variation A1), or alone (variation A2); variation B1 – A. cystica originated from A. gastroduodenalis, or was a branch of the common trunk (R. dexter) (variation B2). The metric data were processed by GraphPad Prism 6 for Windows. Clinically relevant relations between А. сystica, Ductus cysticus, A. celiaca and R. sinister also were described. The new information re-ceived about the blood supply of the gallbladder would contribute to the understanding of the etiology of postoperative complications as a result of surgical interventions in this location and for their prevention.
The study presented in detail the localization and density of mast cells (MCs) in the intramural part of the common bile duct (CBD) and in the major duodenal papilla (MDP) of domestic swine. MCs' density (number/mm(2) ) in different layers of both of the duct and papilla was evaluated after toluidine blue staining. Their number was higher in the lamina propria mucosae than in the tunica muscularis of the studied structures. The localization of berberine-positive, (heparin containing) MCs and the ratio between them and toluidine blue-positive MCs with γ-ma metachromasia was also established. Ratios of heparin-containing MCs in comparison with all toluidine blue-positive MCs were found as follows: ductus choledochus - 32% in the subglandular connective tissue of lamina propria mucosae in the intramural part of the duct; m. sphincter ductus choledochus - 31% in the circular and 0.06% in the longitudinal muscle layer; subserosa - 59%; papilla duodeni major - 0.03% in the subepithelial connective tissue and 34% in the subglandular connective tissue of lamina propria mucosae, respectively. The established large difference in heparin-positive MCs in both the subepithelial and subglandular connective tissues of CBD and MDP, respectively, is an evidence for the existence of mucosal and connective tissue MCs.
Distribution of tripeptidyl peptidase I (TPPI) activity in the structures of porcine lumbar spinal ganglia (LSG) was studied by enzyme histochemistry on cryostat sections from all the ganglia using the substrate glycyl-L-prolyl-L-methionyl-5-chloro-1-anthraquinonyl hydrazide (GPM-CAH) and 4-nitrobenzaldehyde (NBA) as visualization factor. Light microscopic observations showed TPPI activity in almost all the LSG structures. The enzyme reaction in different cell types was compared semi-quantitatively. Strong reaction was observed in the small neurons, satellite ganglia cells and some nerve fibers. Weak reactivity was found in the large sensory somatic neurons, whereas moderate reaction for TPPI was determined in the middle sensory somatic neurons and some nerve fibers. Statistical analysis by one-way ANOVA showed no significance of difference (when p<0.05) for the number of TPPI positive neurons per mm 2 . The original data obtained by the enzyme histochemistry method give us a reason to presume that TPPI actively participates in the functions of all the neuronal structures in porcine LSG. According to our results, it could be suggested that TPPI activity is important for the functions of autonomic and somatic sensory neurons.
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