Heavy metals constitute one of the most important environmental problems. They can be transported in an environment in various forms: emitted into the atmosphere they are carried over long distances, accumulate in soil, sediments and organisms by bioaccumulation, and the biomagnification processes in the trophic chain [1]. Deposition of trace elements and heavy metals in vegetables [2] and mushrooms [3][4][5][6] to a large extent already has been proven, especially in areas potentially contaminated or located in the zone of pollutants, such as power plants, waste dumpsites, metal smelters, and chemical plants [7].Due to the fact that overgrowing topsoil long-lived mycelium can retrieve with water significant amounts of trace elements and pass them to the fruiting bodies of macrofungi [8], there is a risk of bioaccumulation of detrimental elements in organisms of potential consumerswild forest animals, such as roe deer, red deer, wild boars [9], as well as people. The international surveys revealed that macrofungi are able to accumulate in their fruiting bodies a large amounts of cadmium, lead, mercury, zinc, copper, manganese, iron, chromium, silver, and nickel absorbed from contaminated soil [3][4][5][6][10][11][12].The purpose of this study was to determine the level of heavy metals (Pb and Hg) and trace element (Zn, Mn, Cu, and Fe) concentrations in fruiting bodies of 8 species of macrofungi, and to indicate the bioaccumulation factors of elements from soil substrate to fruiting bodies.Pol. J. Environ. Stud. Vol. 24, No. 2 (2015), [651][652][653][654][655][656] Materials and MethodsMushroom and soil samples were collected in October 2010 from Wkrzanska Forest adjacent to Nowe Warpno in the West-Pomeranian voivodeship of north-western Poland (Fig. 1). The main branch of industry in the sampling area is fisheries, thus the forest environment is potentially unpolluted. Macrofungi and soil substrate samples were collected from dry coniferous forest, characteristic for this region. The forest was a monoculture of Pinus sylvestris with a few other species occurring only in undergrowth, like juniper and maple.Generally, 25 samples (each comprising 2-5 fruiting bodies) of eight mushroom species with underneath fruiting bodies soil samples were collected. The examined species were edible Boletus badius (Fr.) Fr. The fruiting bodies were cleaned from any debris, divided into cap and stalk, and then dried in a laboratory oven with a temperature up to 40ºC. Dried samples were homogenized using a laboratory mill. Simultaneously, soil samples were air-dried for several days, sieved with 1 mm pore sieve, and then pulverized using a mortar.Digestion of mushroom samples (0.5 g) was performed with a cold oxi-acidic mixture of 65% HNO 3 and perhydrol (4 and 1 mL, respectively) for at least 12 hours and then put in a MilestoneTM microwave oven. Digestion of soil samples (0.5 g) was conducted using 6 mL of a 5:1 concentrated HNO 3 :HClO 4 (65:60%) mixture and 1 mL of perhydrol and also mineralized in the microwave oven. Mineral...