Objective: The objective of the current study is toward the green synthesis of silver nanoparticles from the aqueous leaf extracts of Piper betle (BL) and to evaluate its anti-diabetic efficacy. Methods: Silver nanoparticles were prepared with the aqueous leaf extract of Piper betle. Characterization of silver nanoparticles prepared from extracts of Piper betle was done by UV-Visible spectrophotometer absorbance spectrum; Scanning Electron Microscope; and Fourier transform infrared spectrophotometer. The anti-diabetic efficacy was ascertained by inhibition assay of α-amylase activity and glucose diffusion inhibitory study with aqueous leaf extract of Piper betle and silver nanoparticles of Piper betle (BLNP). Results: Based on the findings of spectrophotometer studies, it was confirmed that the silver nanoparticles were generated. High-density silver nanoparticles were synthesized by Piper betle was depicted by scanning electron microscope. The plant extracts (BLE) and its nanoformulation (BLNP) showed a constructive impact on controlling the glucose level by the inhibition of α-amylase activity and glucose diffusion. Conclusion: It was concluded that the Piper betle can be employed as alternative medicine in the efficient management of Diabetes Mellitus.
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