Methodology is introduced to separate and quantitate most of the diand trisaccharides composed of glucose and fructose moieties, found in honey. At least twelve closely related disaccharides and seven trisaccharides were successfully identified and quantitated employing a combination of reduction and trimethylsilylation followed by either temperature programmed (disaccharides) or isothermal (trisaccharides) gas chromatography. The oligosaccharides in honey, representing approximately 3% of the total sugars, could be isolated using preparative HPLC and analyzed using the aforementioned gas chromatographic methodology. In addition, these oligosaccharides could also be analyzed without employing a preparative HPLC separation by using a concomitantly larger sample volume. Palatinose, not previously reported in honey, was found in many honey samples.
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