This paper explore the mechanism of lipid extraction efficiency on Moringa oleifera seeds using Soxhlet extraction method. This present study essential to determine the effect of particle size of the sample, extraction time and type of solvent applied towards the efficiency in extracting the lipids from the material. Soxhlet extraction method utilizing Buchi B118 was use in this study and response surface method was applied to analysed the data and determine the optimum parameter condition to obtain the highest yield of Moringa oil extraction. Moringa oil derived from Moringa oleifera seeds was converted into biodiesel (FAME) via Transesterification process. Conversion of Moringa FAME was observed using three different alcohol oil to molar ratio by based-catalysed. This study shows significant strong correlations between particle size of the sample, extraction time and type of solvent use towards extraction yield. The Response surface analysis shows that 1.3611 mm particle size of sample, 3 hours of extraction time and hexane as extraction solvent was the optimum condition in order to get the highest yield of lipids extract from both Moringa oleifera samples. Authentication extraction based on RSM recommendation showed an average Moringa oil yield of 39.75 % by weight.
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