The culture vessels with multiplying shoots of Achras zapota L. on Schenk and Hildebrandt (SH) medium containing 8.88 µM 6-benzylaminopurine (BAP) with or without sucrose were kept under varied CO 2 concentrations ranging from 0.6 to 40.0 g m -3 using different concentrations of sodium bicarbonate (NaHCO 3 ), sodium carbonate (Na 2 CO 3 ), potassium bicarbonate (KHCO 3 ), and potassium carbonate (K 2 CO 3 ) in small acrylic chambers. Complete absence of carbon source caused death of shoots within 20 d. Under elevated concentrations of CO 2 (10.0 and 40.0 g m -3 ) the shoots grew photoautotrophically on sucrose-free medium. The growth of cultures was better at 40.0 g (CO 2 ) m -3 than on 3.0 % sucrose under ambient air of growth room. However, the best response was obtained at 10.0 g (CO 2 ) m -3 and 3.0 % sucrose where maximum number of shoots, shoot length, fresh and dry mass, total number of leaves and leaf area was observed.
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