In vitro Growth and Shoot Multiplication of Achras zapota in a Controlled Carbon Dioxide Environment

Dave, N.; Purohit, S. D.

doi:10.1023/b:biop.0000047164.06779.27

Cited by 5 publications

(2 citation statements)

References 19 publications

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“…[50][51][52] In the present investigation, the combination of 3.0% sucrose and controlled and enriched CO 2 environment proved to be a better option for in vitro shoot growth and multiplication in C. paniculatus. Similar synergistic effects of combinations of sucrose and CO 2 on in vitro shoot growth were observed in tobacco, 53 Wrightia tomentosa, 31 apple, 54 C. papaya 46 and Actinidia deliciosa. 55 Besides this, it was also observed that liquid medium was superior in overall growth over semi-solid medium in CO 2 -enriched conditions.…”

Section: Discussionsupporting

confidence: 66%

“…24,27 Enhanced plantlet growth and multiplication under controlled carbon dioxide enriched environment have been achieved in a number of herbaceous, 28,29 and woody plants. [30][31][32][33][34][35][36] The present study was undertaken hypothesizing that the liquid medium and CO 2 enrichment could synergistically affect the in vitro growth of C. paniculatus, both in terms of rate of multiplication as well as their photosynthetic efficiency. It was also assumed that the plantlets obtained would be of better quality having strong potential of their survival during ex vitro transplantation.…”

Section: Introductionmentioning

confidence: 99%

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Photosynthetic efficiency and in vitro growth of Celastrus paniculatus Willd. under varied concentrations of CO2

Habibi¹,

Purohit²

2019

Int J Phytocos Nat Ingred

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Introduction: Celastrus paniculatus (family: Celastraceae) is a woody climbing shrub valued for its immense medicinal properties contained in its various plant parts. Over-exploitation and poor natural regeneration either by seed or other method/s have resulted into depleting population of C. paniculatus in natural habitats in India. Novel approaches such as liquid culture media and photoautotrophic multiplication of shoots on sugar-free media has proved useful to obtain photosynthetically active micropropagated plantlets. Methods: The shoot cultures of C. paniculatus were multiplied on sucrose containing and sucrose-free semi-solid and liquid media. The cultures were further incubated under various concentrations of carbon dioxide (0.0 to 40.0 gm-3). The assessment of chlorophyll fluorescence parameters (F o , F m , F' m , F t , F v /F m and ΦPS2) under similar conditions was undertaken. Results: It was observed that CO 2 enrichment favored shoot multiplication and elongation and biomass production in sucrose supplemented medium. CO 2 (10.0 gm-3) along with sucrose (3.0%), recorded maximum growth on semi-solid and liquid media. The valuation of F o , F m , F' m , F t , F v /F m and ΦPS2 revealed that an increase in the concentration of CO 2 resulted in a decline in all the parameters especially the F v / F m and ΦPS2. On the contrary, withdrawal of sucrose from the medium under CO 2 enriched conditions resulted in a moderate growth rate and biomass production. However, F v /F m and ΦPS2 were considerably improved in shoot cultures incubated under elevated concentrations of CO 2 (10.0 gm-3) without sucrose in the medium indicating their photoautotrophic growth. Conclusion: Liquid medium proved to be superior for overall growth and biomass production over its semi-solid counterparts. The observations of photochemical efficiency in shoot cultures grown on liquid medium were at par with their semi-solid counterparts indicating no adverse effects such as hyperhydricity.

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Section: Discussionsupporting

confidence: 66%

Section: Introductionmentioning

confidence: 99%

Photosynthetic efficiency and in vitro growth of Celastrus paniculatus Willd. under varied concentrations of CO2

Habibi¹,

Purohit²

2019

Int J Phytocos Nat Ingred

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Potential Role and Utilization of Piriformospora indica: Fungal Endophytes in Commercial Plant Tissue Culture

Tarte¹,

Chandra²,

Dev³

et al. 2022

Commercial Scale Tissue Culture for Horticulture and Plantation Crops

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Effect of controlled carbon dioxide on in vitro shoot multiplication in Feronia limonia (L.) Swingle

Vyas

Purohit

2006

Acta Physiol Plant

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Key words: Fruit tree; in vi tro growth; Photoautotrophic mul ti pli ca tion; Car bon di ox ide en richment Ab stractThe ef fect of con trolled car bon di ox ide en vi ron ment on in vi tro shoot growth and mul ti pli ca tion in Feronia limonia (a trop i cal fruit plant, Fam ily-Rutaceae) was stud ied. Car bon di ox ide available in the am bi ent air of the growth room was in suf fi cient for in vi tro growth of the shoots alone. Also, the pres ence of su crose only as the C-source in the me dium (with out CO 2 ), was found to be in ad e quate for sus tain able growth and mul ti pli ca tion of shoots. The car bon di ox ide en rich ment pro moted shoot mul ti pli ca tion and over all growth. The promotory ef fect of CO 2 was in de pend ent of the pres ence of su crose in the me dium. In the pres ence of both CO 2 and su crose, an ad di tive ef fect was ob served pro duc ing maxi mum shoot growth. In the ab sence of su crose a higher con cen tration of CO 2 (10.0) g·m -3 was re quired to achieve photoautotrophic shoot mul ti pli ca tion com pa ra ble to am bi ent air con trols. High est leaf area per shoot clus ter pro mot ing shoot growth and mul ti pli cation was re corded un der this treat ment. Shoots grow ing on su crose con tain ing me dium un der con trolled CO 2 en vi ron ment of 0.6 g·m -3 con cen tra tion evoked better re sponse than am bi ent air controls (shoots grow ing on su crose con tain ing me dium) in growth room. This treat ment pro duced the over all best re sponse. The pres ent study high lighted the pos si bil ity of photoautotrophic multi pli ca tion which might prove use ful for suc cess ful hard en ing and ac cli ma ti za tion in tis sue cul ture plants.List of ab bre vi a tions: BA -6-benzyladenine, Kn-Kinetin, NAA-α-Napthalene ace tic acid, KHCO 3 -po tas sium bi car bon ate, K 2 CO 3 -potas sium car bon ate, KOH -po tas sium hy drox ide, MS -Murashige and Skoog, Na 2 CO 3 -so dium car bon ate, NaHCO 3 -so dium bi car bon ate, SM -stan dard mul ti pli ca tion me dium, SCSM -sucrose con tain ing stan dard mul ti pli ca tion medium, SFSM -su crose-free stan dard mul ti pli cation me dium In tro duc tionCar bon di ox ide and light are im por tant for growth and de vel op ment of plants (Dur ing and Harst 1996). Dur ing in vi tro cul ture, the growth of chlorophyllous leafy shoots is ad versely affected if ex ter nal source of car bon is not added in the me dium as the CO 2 con cen tra tion in the culture ves sel sig nif i cantly de pletes dur ing light period (Kozai et al. 1992). Al though there is ac cumu la tion of CO 2 in the ves sel dur ing dark pe riod (upto 5-10 mM·mol -1 ) this con cen tra tion soon di min ishes (to less than 100 µM·mol -1 ) with the in cep tion of photoperiod. To com pen sate the require ment of CO 2 , su crose is added in the cul ture me dium as an al ter nate source of car bon in all the 605 ACTA PHYSIOLOGIAE PLANTARUM

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In vitro Growth and Shoot Multiplication of Achras zapota in a Controlled Carbon Dioxide Environment

Cited by 5 publications

References 19 publications

Photosynthetic efficiency and in vitro growth of Celastrus paniculatus Willd. under varied concentrations of CO2

Photosynthetic efficiency and in vitro growth of Celastrus paniculatus Willd. under varied concentrations of CO2

Potential Role and Utilization of Piriformospora indica: Fungal Endophytes in Commercial Plant Tissue Culture

Effect of controlled carbon dioxide on in vitro shoot multiplication in Feronia limonia (L.) Swingle

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