It is shown that microorganisms are an integral element of the mаcroorganism immune system.Peptidoglycan, muramyldypeptyd, teichoic acids are structural components of cell walls of microorganisms. These componentsare an object for recognition of the innate immunity system. The necessity of the bacteria cell walls destruction with a view toobtain the immunotropic products for enteral consumption, able to overcome the intestinal barrier, was substantiated.The use of lactic acid bacteria (ICD) for such purposes is perspective and safe, since the considerable experience oftheir cultivation was accumulated, in addition, ICD have got «GRAS» (Generally Recognized As Safe) status. Waste productsof ICD are organic acids, hydrogen peroxide, bacteriocins and others. These substances have got antagonist activity, implicitlyaffect on the immune system, reducing the antigenic load caused by pathogenic microorganisms.A number of physical, chemical and biochemical methods of bacteria cell walls destruction were considered. Thepriority methods is the soft influence, namely the use of specific enzymes or hydrolases, own autolyzins with a combination ofphysical destruction methods.
The possibility of muropeptides obtaining of peptidoglycans of Lactobacillus delbrueckii subsp. Bulgaricus B-3964 cell walls by the combination of the use of autolytic processes and enzyme treatment of biomass with the participation of lysozyme and papain has been considered. It has been established that the most significant autolytic changes in biomass occur in the application of high-temperature processing (90°C for 30 minutes) in the final stage of the logarithmic phase of bacterial growth. Thus, after eighth hour of biomass incubation at 37°C, the amino acid content in the culture medium was 1.8 mg/cm3, and at 90°C it was 5.7 mg/cm3. In order to further destruction of biomass autolysate and obtaining of low molecular weight peptidoglycan fragments, the process of its enzymatic hydrolysis was studied with lysozyme and papain separately and at their combination. The highest content of low molecular weight peptides in the reaction medium occurred at enzymatic hydrolysis of biomass Lactobacillus delbrueckii subsp. Bulgaricus B-3964 by the composition of enzymes at a ratio of lysozyme : papain 1:2. At a concentration of enzymes 10 mg/cm3, the content of low molecular weight peptides was 7.2 mg/cm3 after eighth hour of incubation of the reaction mixture. The results of studies have been shown that the efficiency of enzymatic hydrolysis of autolysates is much higher. Thus, the amount of low molecular weight peptides in the hydrolysate obtained by processing the autolysate with the composition of lysozyme : papain 1:2 at an enzymes concentration 10 mg/cm3 and the duration of the process for 8 hours by 36% higher than for similar hydrolysis parameters without the use of the process of autolysis.The method of gel chromatography was proved that in the hydrolysate there are fractions of protein compounds with a molecular weight in the range of 70–90 kDa, 30–40 kDa and 294–650 Da. The molecular weight of the latter fraction corresponds to the mass of the muramyl dipeptide. The presence of muropeptides was proved by reaction with the Anthron reagent.
Способ производства пищевого полуфабриката из цельной крови убойных животных [Текст] /Т.Л. Корчагина, В.М. Киселев, Т.М. Шухова (СССР).-№ 467 Б 934 / 13; заявл. 27.01.89; опубл. 30.04.89, Бюл. № 17. 5. Жаринов, А.И. Полифункциональное использование плазмы крови и белоксодержащих систем на её основе в технологии мясопродуктов: дис. … на соискание ученой степени д-р техн. наук [Текст] /А.И. Жаринов.-М., 1991.-498 с. 6. «Гемобин»-противоанемическая биологически активная добавка нового поколения [Текст] /[С.Л.
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