According to the Regulation (EC) of the European Parliament and of the Council of the European Union No. 852/2004 and No. 853/2004 “Оn the hygiene of foodstuffs” dated April 29, 2004, Ukrainian producers of both pasteurized and raw cow's milk should be guided by regulatory acts, in particular, DSTU 2661:2010 “Drinkable cow’s milk. General technical conditions” and DSTU 3662:2015 “Cow’s milk-raw. Technical conditions”, which are harmonized to the requirements of the EU, and are regulating the safety and quality of milk for adhering to the proper sanitary and hygienic requirements of their primary production and industrial processing, and use modern, effective developed and tested rapid control methods of milk and dairy products for their falsification. Taking into account that today the falsification of milk as pasteurized and raw is not a rare occurrence, it is especially important to develop and introduce new express methods for the detection of various chemical reagents in dairy products that impair their quality and can affect their safety. We have developed simple, effective, reliable express methods: the determination of the number of somatic cells in milk by using Reagent N; detection of milk falsification by sodium bicarbonate using an alcoholic solution of bromocresol green with a mass concentration of 0.01%; as well as by alkaline detergents using an alcoholic solution of bromotymol blue with a mass concentration of 0.02%. Determination of the number of somatic cells in cow's milk by express method using Reagent N gave a 99.8% confidence in qualitative indices. The colour and consistency of milk varies, depending on the number of somatic cells – from liquid with bright orange to the formation of a jelly-like clay dark-raspberry colour which is easily removed from the hole of the dairy plate. An express method for detecting falsification of milk by sodium bicarbonate has the following advantages over existing commonly used methods: the results are specific, with a reliable qualitative index of 99.9%, consisting in changing the colour of the circular layer of milk, depending on the amount of baking soda added to it. In the absence of sodium hydro-carbonate, the colour of the circular layer of milk has a dark green colour, and if it is present, it acquires a dark blue-green colour of varying intensity. The express method of the milk falsification determining by alkaline detergents is easy to implement, and its results give specific qualitative indicators of 99.9%. Depending on the amount of alkaline detergents added to the container with milk (up to 1, 5, 10%), the milk acquires a blue colour varying intensity.
Визначено безпечність та якість молока різних молокопереробних підприємств, яке використовується для харчування населення України. Встановлено, що за органолептичними показниками, густиною, кислотністю молока, вмістом жиру, білка, сухого знежиреного залишку, сухої речовини, бактеріального обсіменіння молоко торгових марок «Віта» (ПАТ-ЖЛК «Україна»), «Яготинське» та агропромислового ринку м. Біла Церква Київської області, відповідало вимогам національного стандарту України (ДСТУ 3662:2015). Молоко торгових марок «Віта» (ПАТ-ЖЛК «Україна», «Яготинське» за чистотою молока (кількість механічних домішок), фальсифікацією (домішки води) та забрудненням лужним миючим засобом – до 5% не відповідало вимогам чинного ДСТУ 3662:2015. Вперше застосовано спосіб визначення фальсифікації молока натрію гідрокарбонатом із застосуванням бромкрезолового зеленого (Патент України на корисну модель № 118246; 2017 р.). Вперше застосовано спосіб для визначення кількості соматичних клітин у молоці (наявність субклінічної форми маститу) із застосуванням розчину Reagent N (Австрія) (Патент України на корисну модель № 118247; 2017 р.). Запропоновані способи достовірні (99,2–99,8%), ефективні, експресні, зручні в проведенні, не вимагають витрат на реактиви і можуть використовуватися в комплексі з іншими методами визначення безпечності та якості молока.
The work was performed under conditions of JSC «Gorodenkovskiy cheese» Ivano–Frankivsk region. Ob'yektom study was raw milk that came from different entities. Milk studied organoleptic and laboratory methods for conventional methods. Navedeni results of the study parameters of quality and safety of milk, which reported on molokokopererobne company from different entities, collective farms, private farms. The studies have shown that milk from entities in milk business reported first grade from private farms – the second, as evidenced by indicators titrated acidity – 17.5 ± 0.56 ºT and 19.3 ± 0.4 ºT respectively. Milk from individual farms to milk processing company reported non–refrigerated – 14.1 ± 0.5 ° C, cooled entities with an average temperature 8.3 ± 0.1 °C, which is likely to affect its quality. Density commodity raw milk obtained from farms of different ownership did not differ significantly (P < 0.05) fat 3.5 ± 0.25%, protein and 3.0 ± 0.07, than in samples obtained from entities that can be oznakoyuyoho alleged fraud, or increased stress on the metabolism. To guarantee the safety of milk producers should be used as raw material control system at all levels of the food chain, starting with control of mineral fertilizers and plant protection products in pastures, sources of water intake, health and welfare and finishing, storage and transportation milk processing enterprises
The article is devoted to the study of the morphological features of the skull of dairy cattle depending on the sex in a wide age range (from birth to 10 years) at the different levels ofstructural organization. It has been established that the assessment of the informative quantitative parameters of the skull (craniometric, roentgenodensitometric, spectral) and the formalization of its qualitative characteristics (cranioscopic, roentgenomorpho- logical) allows to determine the age of the cattle comprehensively, objectively and trustworthy on the basis of the reference-test principle. The latter one is the basis for the computer program "Cattle Skull", which automatizes the developed methods of cattle age and sex determination by the skull and eliminates the errors during the processing of the research results, it improves the accuracy and objectivity of the craniological examinations. According to the results of the multifactorial dispersion analysis, to determine the age and sex of the cattle is influence: according to linear craniometric indices - the age of the animals (81-100 %), sex (0-17 %) and random factors (0-6 %); according to the results of IR-spectroscopic research - random factors (49-59 %), age (36-50 %) and sex (1-4 %); by Hounsfield parameter (HU) - age (82-96 %), sex - (1-16 %) and random factors (0-2 %); according to computer tomographic and densitometric indices (CTDI) values - age (5-82 %), sex (2-89 %) and random factors (6-51 %). Osteological technology, in the basis of which there is laid consideration of the integrity of the skull, the quantity and complexity of applied methods of skull research, allows to determine the age of the dairy cattle by the qualitative and quantitative parameters of the skull from birth to the age of 10 years with an accuracy of 2-10 months.
Bacterial safety in the modern poultry industry plays a key role and is one of the key factors in production effi ciency. In the structure of poultry infectious pathology, the leading place is occupied by such bacteria as Escherichia coli, Clostridium perfringens, Enterococcus cecorum, Staphylococcus aureus, Gallibacterium anatis. The results of studies of samples of pathological material obtained from a sick bird are presented that indicate that most often Escherichia coli cultures were isolated from the heart (41,5 %), liver (22,0 %) and lungs (20,7 %), less often spleen (5,2 %) and kidney (2,0 %). Most isolated cultures of Escherichia coli (78 %) caused hemolysis when plating material on blood agar. The largest number of pathogenic cultures of Escherichia coli was isolated from adult chickens, signifi cantly fewer chickens under the age of 20 days. The associated course of bacterioses caused by two or more pathogens was noted in 89,8 % of cases. In 38,5 % of cases, Escherichia coli, bacteria of the genus Staphylococcus and Gallibacterium anatis were isolated from pathological material, in 27,3 % – a joint course of escherichiosis, staphylococcosis and enterobacteriosis was noted, in 15,7 % – escherichiosis, salmonellosis and enterobacteriosis, in 8,3 % – pasteurellosis and enterobacteriosis. In 13,2 % of cases with pathological material from chickens (liver, joints, in chickens – blind processes of the intestine) Clostridium perfringens was isolated. Three species of bacteria of the genus Staphylococcus were identifi ed: Staphylococcus aureus, Staphylococcus chromogenes, Staphylococcus pluranimalium (51,7 % from the liver, 21,7 from the lungs, 18,3 from the spleen, 5,0 % from the kidneys). Enterococcus cecorum was identifi ed in 11,32 % of the studied samples from cloacal swabs, oviducts and bone marrow, and Gallibacterium anatis was identifi ed in 11,32 % (from the upper respiratory tract and genitals). Key words: poultry farms, monitoring, bacterial infection, Escherichia coli, Staphylococcus aureus, Staphylococcus chromogenes, Staphylococcus pluranimalium, Enterococcus cecorum, Clostridium perfringens, Gallibacterium anatis.
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