The medial superior olive (MSO) is an important brain center that computes sound location by comparing small differences in arrival time at the two ears. The MSO is investigated for its specializations for processing with extreme temporal precision. In mice, the MSO is small and difficult to study; thus utilization of genetic methods in MSO studies has been lacking. We present the first comprehensive study of the murine MSO and show that most features are consistent with more heavily investigated species.