Objective: The treatment of chronic wounds is one of the actual problems in medicine. Photobiomodulation (PBM) has gained considerable recognition among treatment modalities for various medical problems including wound repair processes. This research aims to investigate the role of intercellular mediators in the regulation of reparative processes of chronic wounds with the PBM therapy in the experiment. Methods: Studies were performed on 12 Wistar rats weighing 250 ± 30g. Rats were used for modeling a chronic wound. Animals were divided into two groups: control and experimental.The animal wounds from the experimental group were treated with low-intensity laser radiation from “Lika-therapist М” in continuous mode using a wavelength of 660 nm, output power at 50 mWt, energy density at 1 J/cm² once per day for 5 days. On the 14th day after the application of wounds, 6 rats from each group were removed from the experiment. PBM efficiency was evaluated with intercellular mediators analysis, as well as with histological examination. ELISA serum analysis was done for quantifying interleukin- 1β (IL-1β), tumor necrosis factor alpha (TNF-α), interleukin-4 (IL-4), interleukin-6 (IL-6), interleukin-10 (IL-10) and granulocyte macrophage colony stimulating factor (GM-CSF). Statistical processing of the results was performed using the Statistica 6.0 analysis package. Result: PBM effect on the expression of intercellular mediators in blood serum of animals with chronic wounds after 14 days after wound modeling, showed: increase of cytokine IL-1β (p > 0,05) and IL-4 (p < 0,05) levels; decrease of TNF-α and IL-10 levels (p < 0,05); without significant changes were concentrations IL-6 and GM-CSF. The histological study showed decrease vascular count and better organization of collagen fibers in experimental group. Conclusion: Histological studies of wound healing revealed an earlier transition of the process to the remodeling phase with the use of PBM therapy. PBM therapy can be an instrument for optimizing the reparative process by correcting the regulation by intercellular mediators. Bangladesh Journal of Medical Science Vol. 21 No. 02 April’22 Page : 398-403
BACKGROUND: In patients with a fracture of the proximal femur and concomitant arterial hypertension, there is a disturbance of the reparative processes of bone tissue. This research aimed to study the regulation of the reparative processes of fractures of the proximal femur with intramedullary osteosynthesis during the correction of concomitant hypertension, which was examined based on some markers using the rat model.METHODS: The study involved healthy Wistar rats and spontaneously hypertensive rats (SHR). The subjects were then grouped into healthy rats without exposure (1.1) SHR without exposure (2.1), healthy rats with modeled fractures of the proximal femur (1.2), SHR with modeled fractures of the proximal femur (2.2), SHR underwent hypertension correction with enalapril in subgroups without fracture (2.3) and SHR underwent hypertension correction with enalapril in subgroups with fracture (2.4). The levels of interleukin (IL)-6, tumor necrosis factor alpha (TNF-a), IL-10, amino-terminal propeptide procollagen type III (PIIINP), glucose, uric acid, creatinine, urea, cholesterol, and albumin were determined in the blood serum of the animals. Femur preparations were examined after the removal of intramedullary fixation. RESULTS: Serum IL-6 level of animal in group 2.4 (2.297±0.361 pg/mL) were reduced compared to the corresponding indicators of rats in group 2.3 (4.054±0.491 pg/mL, p<0.05). Serum glucose and urea levels of animal in group 2.4 (3.951±0.156 mmol/L, 6.552±0.426 mmol/L, respectively) were significantly reduced in comparison with the group 2.3 (6.384±0.890 mmol/L, 10.369±0.888 mmol/L, respectively). The histological results indicated a positive effect of the drug enalapril on the healing of fractures of the proximal femur in animals with hypertension.CONCLUSION: Correction of arterial hypertension with enalapril in fractures of the proximal femur improves the reparative processes of bone tissue.KEYWORDS: injury healing, remodeling, concomitant diseases, angiotensin-converting enzyme inhibitors, cytokines, growth factor, collagen, biochemical parameters
Treatment of patients with fractures of the proximal femur is an important problem in modern traumatology. Hip fractures are more commonly associated with osteoporosis. Elderly and senile people make up a significant proportion of patients with fractures. Arterial hypertension (AH) in the elderly population is a disease with a high prevalence. When treating fractures of the proximal femur, it is necessary to take into account the features of reparative osteogenesis characteristics of patients with concomitant arterial hypertension. Medicines used to treat hypertension have a beneficial effect on bone tissue. Pharmacological correction of hypertension in these patients is essential to optimize fracture healing.
Стаття надійшла до редакції 21.06.2019 ДО УВАГИ СПЕЦІАЛІСТІВ ДУ «Інститут патології хребта та суглобів ім. проф. М. І. Ситенка НАМН України» проводить післядипломну підготовку фахівців на курсах інформації та стажування з актуальних питань ортопедії та травматології (ліцензія Міністерства освіти і науки України АЕ № 285527 від 27.11.2013
Introduction: Treatment of chronic wounds is an urgent worldwide problem for medicine. Among the many efforts in wound healing techniques, photobiomodulation therapy has shown promising results. Aim: To study the effect of photobiomodulation therapy on optimisation of the regulation of reparative processes in chronic wounds by cytokines (through the example of interleukin-1β, tumour necrosis factor-alpha, interleukin-6, interleukin-4, interleukin-10, and granulocyte macrophage colony stimulating factor). Materials and methods: The study involved 12 Wistar rats. A trophic wound was modelled in the animals. After the operation, the animals were divided into two groups of 6 animals each. The wound defects of animals in the experimental group were exposed to low-intensity laser radiation. Photobiomodulation therapy was applied once a day for 5 days, starting the day after wound modelling. The device Lika-therapist M (Ukraine) was used in a continuous mode at a wavelength of 660 nm, an output power of 50 mW, and an energy density of 1 J/cm², and 60 s exposure time. Results: The study showed the following changes in the expression of intercellular mediators in the blood serum of animals with chronic wounds when using photobiomodulation therapy: a decrease in the levels of interleukin-1β (p>0.05) and tumour necrosis factor-alpha (p<0.05); increased concentrations of interleukin-4 (p<0.05); the concentrations of interleukin-6, granulocyte macrophage colony stimulating factor, and interleukin-10 were not significantly changed. The histological study showed better organization of collagen fibers in the experimental group. Conclusions: Photobiomodulation therapy can be an instrument for optimizing the reparative process by correcting the regulation by intercellular mediators.
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