<p>Layu yang disebabkan oleh Fusarium spp. merupakan salah satu penyakit penting tanaman stroberi (Fragaria x ananassa<br />Dutch.) di daerah subtropika, yang dapat menggagalkan panen. Penelitian bertujuan untuk mempelajari potensi Trichoderma spp.<br />dalam mengendalikan Fusarium spp. Isolat Trichoderma spp. diisolasi dari rizosfer tanaman stroberi dan Fusarium spp. diisolasi<br />dari tanaman stroberi yang mengalami layu fusarium. Isolat cendawan dimurnikan, dikarakterisasi, dan dibandingkan dengan isolat<br />cendawan acuan. Uji antagonis dilakukan secara in vitro dan in vivo. Uji in vitro dilakukan dengan metode dual culture dan slide<br />culture. Uji in vivo dilakukan di rumah kasa menggunakan dua varietas stroberi, yaitu Santung serta California. Hasil penelitian <br />in vitro memperoleh dua jenis isolat cendawan antagonis, yaitu Trichoderma sp.1 dan Trichoderma sp.2, dan dua jenis cendawan <br />patogen Fusarium, yaitu Fusarium sp.1 dan Fusarium sp.2. Isolat Trichoderma sp.1 memiliki kemampuan antagonisme lebih tinggi<br />dibandingkan dengan isolat Trichoderma sp.2. Isolat Trichoderma sp.1 mampu menghambat pertumbuhan Fusarium sp.1 dan<br />Fusarium sp.2 secara berturut- turut, yaitu 49,7% dan 49,6%. Isolat Trichoderma sp.2 mampu menghambat pertumbuhan Fusarium<br />sp.1 dan Fusarium sp.2 lebih rendah, yaitu sebesar 45,8% dan 43,4%. Mekanisme antagonis yang terjadi antara cendawan antagonis<br />dan patogen pada uji in vitro, yaitu pembelitan dan intervensi hifa. Hasil pada uji in vivo pada perlakuan Trichoderma sebelum<br />Fusarium menunjukkan keefektifan pengendalian paling baik (41,72%) dibanding perlakuan lain. Varietas Santung lebih tahan<br />terhadap serangan patogen dibandingkan varietas California. Implikasi dari hasil penelitian ini adalah, agens hayati Trichoderma<br />spp. lebih optimal digunakan sebagai pencegahan (preventif) tanpa menunggu tanaman terinfeksi penyakit layu fusarium.</p>
Soil microorganisms such as Trichoderma asperellum and Gliocladium sp have the potential to become biological control agents in inhibiting the growth of fungi Botryodiplodia theobrome which causes stem rot disease in citrus plants. The purpose of this study was to determine the potential of T.asperellum and Gliocladium sp fungi in inhibiting the growth of B. theobrome fungus causing stem end rot disease in citrus plants. The antagonistic test was carried out using the dual culture method using PDA media; observations were made on the growth of pathogenic fungi and antagonistic fungi to measure their inhibitory power. The results of the observation and calculation of the fungal growth rate were analyzed using the T test with a level of 5%. The pathogen fungal growth rate in the antagonistic test was slower than control and inhibition of B. theobrome by T.asperellum and Gliocladium sp were respectively at 78.67% and 84.56%. The mechanism of the two antagonistic fungi has the nature of competition and parasitism because of the broader inhibition spectrum than the pathogenic fungi. Keywords: Botryodiplodia theobromae, Trichoderma asperellum, Gliocladium sp, antagonistic test.
<p>Penyakit diplodia (Botryodiplodia theobromae) pada tanaman jeruk menyebar cukup luas di sentra jeruk Indonesia. Serangan parah penyakit dapat menyebabkan kematian apabila tidak dikendalikan. Tujuan penelitian adalah mengetahui patogenisitas dan peran toksin dari tiga isolat B. theobromae asal Pasuruan dan Magetan pada jeruk siam, pamelo, dan manis. Penelitian dilakukan di Laboratorium dan Rumah Kasa Balai Penelitian Tanaman Jeruk dan Buah Subtropika pada bulan November 2015 – Mei 2016. Penelitian terdiri atas dua percobaan, yaitu uji patogenisitas pada tanaman dan uji toksin kasar pada skala laboratorium. Uji patogenisitas menggunakan rancangan acak kelompok dengan sembilan kombinasi perlakuan terdiri atas tiga jenis isolat, yaitu Mg52A.1, dan Mg39.2 (asal Magetan), Ps8b (asal Pasuruan), serta tiga jenis tanaman jeruk (pamelo, siam, manis). Parameter pengamatan terdiri atas masa inkubasi, jumlah sampel nekrosis, dan luas gejala. Perlakuan pengujian toksin terdiri atas kontrol tanpa toksin, toksin kasar isolat Mg52A.1, toksin kasar isolat Mg39.2, dan toksin kasar isolat Ps8b. Aplikasi toksin dilakukan pada daun tiga varietas jeruk dengan rancangan acak lengkap, tiap perlakuan diulang tiga kali dan masing masing terdiri atas dua daun asal tanaman yang berbeda. Hasil penelitian menunjukkan bahwa masa inkubasi isolat Mg39.2 lebih cepat dibandingkan dengan isolat Mg52A.1 dan Ps8b. Ketiga isolat patogen B. theobromae asal Pasuruan dan Magetan memiliki patogenisitas yang sama dalam menimbulkan gejala penyakit pada jeruk pamelo, siam, dan manis, sedangkan toksin hanya berperan dalam mempercepat masa inkubasi.</p><p>Diplodia disease (Botryodiplodia theobromae) spread quite widely in Indonesia citrus center. Severe attacks of disease can cause death if it not controlled. The purpose of this study was determine the pathogenicity and the effect of toxins from three isolates of B. theobromae origin Pasuruan and Magetan on tangerine, pummelo, and sweet orange varieties. The study was conducted at Indonesian Citrus and Subtropical Research Institute during November 2015 – May 2016. This observation consisted of two experiments that pathogenicity test in screenhouse and crude toxin of patogen test in laboratory. Pathogenicity test used randomized block design arranged as factorial. The first factor was three isolates: Mg52A.1, Mg39.2 (from Magetan), Ps8b (from Pasuruan) and the second factor were kind of citrus (pummelo, tangerine , and sweet orange). The observation parameter consist of the incubation period, the number of necrotic samples and visual symptom. Crude toxin test treatment consists of a control test toxin without toxins, crude toxin Mg52A.1, crude toxin Mg39.2 toxin, crude toxin Ps8b. Application toxin carried out on the three leaf varieties of oranges. Each treatment was repeated three times and each consists of two leaves of different varieties. The results showed that the incubation period Mg39.2 isolates faster than two other isolates. Infection with different isolates and treatment of different citrus varieties shows that it did not different significantly in causing disease symptom of diplodia. Similarly result on crude toxin treatment with three isolates on three varieties showed that it were not different necrotic symptom. Thus the three isolates of pathogens B.theobromae origin from Pasuruan and Magetan have the same pathogenicity in causing disease symptoms in citrus pummelo, tangerine, and sweet orange. Toxin only play a role in accelerating the incubation period.</p>
Foot Rot (Phytophthora spp.) is an important disease in Indonesia and in the world. Around 35% of citrus plantations have been damaged by the complex of foot rot disease and Diplodia disease (Botryodiplodia theobromae), mainly in swamps and irrigated fields. The spread of the disease is fast, but the knowledge of citrus farmers is still limited, causing rapid death in production plants. This paper purpose is to provide an overview of the citrus root disease control from research studies supporting the efforts of Good Agricultural Practices in Indonesia. Usually farmers only rely on systemic pesticides to control disease, but it is not always successful. Control by means of healthy cultivation is highly recommended in supporting green agroindustry and saving nature. Integrated and rational control strategies, require knowledge of the ecology and epidemic of diseases ranging from seedlings to gardens. Propagule pathogens can survive in seeds, tree stumps, soil, even in alternative hosts. Therefore, to control the disease it must be directed not only to oranges but also to alternative hosts as well and to destroy pathogenic retention sites. The recommended integrated control strategy is to use disease-resistant plants, making sewers, garden sanitation, and optimal maintenance. This method is very effective and environmentally friendly.
Citrus stem rot disease caused by Lasiodiplodia theobromae (Pat.) Griff. & Maubl is a serious disease in citrus orchards in Indonesia. The geographical distribution of these diseases is very broad, almost found in all citrus centers of more than 22 provinces, districts, and cities. Plants of more than 10 years with less intensive maintenance often seriously affected. Alertness and control must be more intensive in productive plants aged more than 5 years old. The disease epidemic problem occurs because of the presence of sensitive citrus varieties, poorly maintained plants, the source of inoculum throughout the year, wide range of host plants, and contamination of agricultural equipment. Sensitive plants in citrus genus including pummelo, orange, tangerine, lime, and lemon. The control strategy is formulated by considering the effectiveness, logical and environmentally friendly to support the development of citrus areas in 24 main citrus centers in Indonesia. Stem rot control strategies include: (1) monitoring of disease incidents in the field, presence of inoculum sources in citrus plants and other hosts, (2) maintaining the cleanliness of the field, sanitation by pruning the branches, (3) optimum maintenance, (4) disinfecting agricultural tools (pruning shears, grafting knives, machetes, saws) with 70% alcohol, (5) Weaning of stems with sulfur lime 2 times a year, (6) Biological control (Trichoderma harzianum, T. asperellum, T. viride, Bacillus subtilis, Gliocladium sp) and or botanical control using cashew nut shell waste (Anacardium occidentale L.), red ginger extract (Zingiber officinale var. rubrum).
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