Potential of Antagonistic Fungi in Inhibiting the Growth of Botryodiplodia theobromae Fungi Causes Stem Rot Disease in Citrus

Abstract

Soil microorganisms such as Trichoderma asperellum and Gliocladium sp have the potential to become biological control agents in inhibiting the growth of fungi Botryodiplodia theobrome which causes stem rot disease in citrus plants. The purpose of this study was to determine the potential of T.asperellum and Gliocladium sp fungi in inhibiting the growth of B. theobrome fungus causing stem end rot disease in citrus plants. The antagonistic test was carried out using the dual culture method using PDA media; observations were made on the growth of pathogenic fungi and antagonistic fungi to measure their inhibitory power. The results of the observation and calculation of the fungal growth rate were analyzed using the T test with a level of 5%. The pathogen fungal growth rate in the antagonistic test was slower than control and inhibition of B. theobrome by T.asperellum and Gliocladium sp were respectively at 78.67% and 84.56%. The mechanism of the two antagonistic fungi has the nature of competition and parasitism because of the broader inhibition spectrum than the pathogenic fungi. Keywords: Botryodiplodia theobromae, Trichoderma asperellum, Gliocladium sp, antagonistic test.

Isolation and antagonistic test of fungi associated with pummelo citrus against Botryodiplodia theobromae in vitro

“…The growth rate of B. theobromae in the control was seen to be faster than in the fungus treatment, so that it can be seen that the isolated fungus from pummelo citrus was able to inhibit the growth of B. theobromae. The growth rate of antagonistic fungi is an indicator that fungi are able to compete with pathogenic fungi, the faster the growth of antagonistic fungi, the more effective it is in suppressing the pathogens growth [17]. The ability to inhibit the development of pathogens is a requirement for an organism to be a biocontrol agent [11].…”

“…4 because they were covered by antagonistic fungal mycelium. The antagonistic fungi that are able to cover pathogenic fungal colonies is referred to as parasitism [17]. Observations on the contact area of the hyphae showed that the antagonist fungal hyphae entwined the pathogenic hyphae and lysis in figure 4.…”

Analysis of stem rot disease severity on Pomelo (Citrus maxima) plantation in Pangkep Regency, South Sulawesi

“…The part of the skin that is attacked by the pathogen will dry out and peel off. Stem rot develops encircling the trunk and branches which can cause death [4].…”

The In Vitro Antifungal Impact of Secondary Metabolites from Trichoderma sp. on Fusarium sp.

“…Thus, innovation is needed in utilizing biological control agents to reduce chemical pesticide usage. A safe control method is using bio-agents that do not pollute the environment (Agustina et al, 2019).Trichoderma is a fungus with potential applications as a natural agent for controlling pathogens. Trichoderma sp.…”

“…Thus, innovation is needed in utilizing biological control agents to reduce chemical pesticide usage. A safe control method is using bio-agents that do not pollute the environment (Agustina et al, 2019).…”