The current study aimed to reduce the toxic effect of different mycotoxins and enhance the immunity against ND virus in broiler chickens by using lymphokines from hyperimmunized birds with Salmonella typhymurium. The study included three stages, the first stage included isolating Salmonella typhymurium. The second stage was immunized chicks with Salmonella typhymurium. The final stage of the study was accomplished by treating 250 broiler chicks (divided into 5 groups, 50 chicks /each) with the following treatments; G1: 0.5 ml lymphokines was injected I/P at day one old with live ND vaccine (la Sota strain) after 30 minutes in drinking water, the process repeated after 10 days; G2: the same as in G1 but inactivated killed vaccine was used s/c,no repetition was carried out at 10 days ; G3: a combination of G1 and G2 with revaccination of live La Sota vaccines only at 10 days; G4 : only vaccinated with live La Sota vaccine repeated at 10 days; G5: no treatments (negative control). All groups were challenge with local isolate of NDV (100ELD50 10 5) at 25 days, all groups except the fifth group were fed on contaminated diet with mycotoxin. The results of the present study showed a significant increase (P <0.05) in antibodies titre against ND in the third group, followed by the first and second groups Measured by ELISA and hemagglutination (HI) test, A significant decrease (P >0.05) in the oxidation status (H2O2, MDA and LPO) and significant increase in the antioxidant defense (GSH-PX) in the liver and spleen samples. We conclude from the current study that the Salmonella immune lymphokines (SIL) helps in enhancement the level of immunity against Newcastle disease and n reduction the side effects of which mycotoxin.

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     Newcastle disease is one of serious pathological problems and causes of vast economic losses during 2011-2016 in Iraq. The disease caused high mortalities in all types of poultry nevertheless of vaccination. In this study all samples were collected from infected flocks with clinical signs of the disease. Inoculation of chicken embryonated eggs was carried out for virus isolation, identification, Haemagglutination and Haemagglutination Inhibition assay. Using Reverse Transcriptase-Polymerase Chain Reaction to confirm the presence of the virus, Intra Cerebral Pathogenicity Index and Mean Death Time were used to confirm all the isolates that were velogenic. The important determinant of Newcastle disease virus pathogenicity is fusion protein that has been used for phylogenetic analysis. sequencing and compared genetically of Newcastle disease virus Iraqi isolate to publish sequences acquired from GenBank showed 99% sequence similarity to the Iran isolate IRI 1392k (KJ176996.1). It can concluded from these data that introduction new virus was occurred in Iraq.

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