Aim:The aim of this in vitro study was to evaluate and the compare antibacterial efficacy of four dentin bonding system against Streptococcus mutans, Streptococcus salivarius and Lactobacillus acidophilus over a period of three months using agar disk diffusion test.Methodology:All the three standard bacterial strains were inoculated into BHI broth and incubated at 37°C for 48 hours. A 100 μl of broth suspension containing aliquots of S. mutans, S. salivarius and L. acidophilus were spread onto M-H agar medium using sterile cotton swabs. The experimental groups were as follows: GROUP A: Test conducted for evaluation of antimicrobial efficacy against S. mutans MTCC 497, GROUP B: Test conducted for evaluation of antimicrobial efficacy against S. salivarius MTCC 1938, and GROUP C: Test conducted for evaluation of antimicrobial efficacy against L. acidophilus MTCC 447. For sample preparation, 20 μl bonding agent was dropped with micropipettes on paper disks, and blown dry for 10 seconds. Then it was light-cured at 2 mm for 20 sec using a QTH visible light curing unit. For first reading, the sample disks were placed over the freshly inoculated agar plates and then incubated at 37°C for 48 hours. The rest of the paper disks were stored in dark, submerged in distilled water at 37°C. They were placed on freshly inoculated spread plates after specific time intervals- 1 day, 1 week, 2 weeks, 1 month and 3 months after ageing in PBS. After incubation, the diameters of zones of inhibition around the plates were measured. The experiment was performed twice in triplicate. The data was then statistically analysed using Two way ANOVA test and post hoc.Results:Results showed that Xeno III had the maximum antibacterial efficacy over a period of three months, followed by XP bond. This antibacterial activity was maximum against Streptococcus mutans, followed by Lactobacillus acidophilus and least against Streptococcus salivarius. Adper Easy One and G bond had minimal effect against the test bacteria during the test period.Conclusion:The antibacterial effect decreased over a period of three months for all the dentin bonding systems.
Aim: The aim of this study was to compare push-out bond strength of root canal spaces filled with AH-Plus and BioRoot RCS after using different irrigants. Materials and Methods: Eighty single-rooted teeth were decoronated and endodontically treated by crown-down technique using ProTaper Universal rotary files progressively till F4 (40/0.06). Samples were divided into two groups according to the sealer ( n = 40): Group A = BioRoot RCS, Group B = AH Plus, further subdivided into four subgroups according to irrigation protocol ( n = 10): Group 1A, 1B – 0.9% saline, Group 2A, 2B – 5.25% NaOCl + 17% EDTA, Group 3A, 3B– 17% EDTA +2% chlorhexidine, and Group 4A, 4B – 17% EDTA + 3% green tea extract. Samples were obturated with sealers and prepared for push-out test with root slices of 2 mm thickness using universal testing machine. Data were statistically analyzed using two-way ANOVA, Bonferroni's post hoc analysis, and independent Student's t -test. Results: BioRoot RCS exhibited significantly higher push-out bond strength ( n < 0.001). The interaction between irrigants and sealer showed higher bond strength for BioRoot RCS when 17% EDTA+ green tea was used. Conclusion: Within the limitation of the study, it was concluded that bond strength of a sealer was influenced by the irrigants used.
Molar incisor hypomineralization (MIH) is a developmental defect affecting teeth. High prevalence rates of MIH and its clinical implications are significant for both the patients and clinicians. A wide variation in defect prevalence (2.4–40.2%) is reported. It seems to differ with regions and various birth cohorts. Some of the recent prevalence studies are tabulated. Patient implications include hypersensitive teeth, rapid progression of caries, mastication impairment due to rapid attrition, and esthetic repercussions. Implications for clinicians include complexity in treatment planning and treatment implementation, poor prognosis of the restorations, difficulty in achieving pain control during treatment, and behavior management problems. Intention of this paper is to review the etio-pathogenesis, prevalence, clinical features, diagnostic features, and eventually present a sequential treatment approach, i.e., in accordance with current clinical practice guidelines. How to cite this article Rao MH, Aluru SC, Jayam C, Bandlapalli A, Patel N. Molar Incisor Hypomineralization. J Contemp Dent Pract 2016;17(7):609-613.
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