The cortical thick ascending limb (CTAL) absorbs Cl Ϫ via a Na ϩ -K ϩ -Cl Ϫ cotransport at the apical membrane and several Cl Ϫ channels at the basolateral membrane, including a 9-pS channel having several properties of the cystic fibrosis transmembrane conductance regulator (CFTR). Having checked that CFTR mRNA is present in the mouse CTAL, we investigated whether this channel is a CFTR molecule by applying the patch-clamp technique to CTALs microdissected from CFTR knockout mice ( cftr m1Unc ).
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