Bifidobacterium breve significantly reduces cigarette smoke-induced COPD in C57Bl/6 mice

Carvalho, Jomar Rezende; Sá, Ana Karolina; Britto, Aurileia; Ferreira, Munique; Anatriello, Elen; Keller, Alexandre C.; Aimbire, Flávio

doi:10.1183/13993003.congress-2018.pa4244

Cited by 1 publication

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“…Recently, it was reported that metabolites of probiotics carried through the circulation to the lungs might help with COVID-19 by inhibiting viral replication or enhancing the immune response [23]. This inhibition of inflammation has been demonstrated in animal studies that induced COPD with DPM and cigarette smoke; Bifidobacterium breve and Lactobacillus rhamnosus reduced gene expression related to mucin production and inflammatory factors, NF-κB, and cytokines [18,24]. Lactobacillus casei HY2782 and Bifidobacterium lactis HY8002 decreased oxidative stress and pro-inflammatory cytokines in the lung in vivo [25].…”

Section: Discussionmentioning

confidence: 95%

Cell-Free Supernatant from Lactobacillus and Streptococcus Strains Modulate Mucus Production via Nf-κB/CREB Pathway in Diesel Particle Matter-Stimulated NCI-H292 Airway Epithelial Cells

Lee¹,

Kang²

2022

Molecules

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Airway epithelial cells are a major site of airway inflammation and may play an important role in the pathogenesis of chronic obstructive pulmonary disease (COPD). Diesel particulate matter (DPM) is associated with mucus hypersecretion and airway inflammation and has been reported to overexpress airway mucin in the NCI-H292 airway epithelial cells. Therefore, regulation of mucin hypersecretion is essential for developing novel anti-inflammatory agents. This study aimed to investigate the effects of cell-free supernatant (CFS) from Lactobacillus and Streptococcus on nitro oxide (NO) production in RAW264.7 and proteins associated with mucus production in NCI-H292 cells. We observed that NO production was reduced by CFS from Lactobacillus and Streptococcus in RAW 264.7, and MUC4, MUC5AC, and MUC5B gene expression was increased by phosphorylation of nuclear factor kappa B (NF-κB) p65 and cAMP response element-binding protein (CREB) in DPM-stimulated NCI-H292 cells. However, CFS from L. paracasei MG4272, MG4577, L. gasseri MG4247, and S. thermophilus MG5140 inhibited mRNA expression related to mucus production by downregulating the CREB/NfκB signaling pathway. These results suggest that CFS from L. paracasei MG4272, MG4577, L. gasseri MG4247, and S. thermophilus MG5140 can contribute as a strategic candidate to the prevention of airway inflammatory diseases caused by DPM.

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