Rice performance under drought stress is mainly impeded by oxidative damage and hampered plant water status, which may be improved by exogenous use of osmoprotectants. In this study, the role of glycinebetaine (GB) to improve drought tolerance in rice (Oryza sativa L.) cultivar Super‐basmati was evaluated. GB was used both as seed and foliar application. For priming, seeds were soaked in 50, 100 and 150 mg l−1 aerated solution of GB for 48 h. At four‐leaf stage, one set of plants was subjected to drought stress, while the other set kept at full field capacity. Drought was maintained at 50 % of field capacity by watering when needed. For exogenous application, 50, 100 and 150 mg l−1 GB levels were applied at five‐leaf stage. Drought stress greatly reduced the rice growth while GB application improved it both under well‐watered and drought conditions. Drought tolerance in rice was strongly related to the maintenance of tissue water potential and antioxidant system, which improved the integrity of cellular membranes and enabled the plant to maintain high photosynthesis. Foliar treatments were more effective than the seed treatments, while among the GB treatment, foliar application with 100 mg l−1 was the most effective.
Water shortage is a severe threat to the sustainability of crop production. Exogenous application of glycinebetaine (GB) and salicylic acid (SA) has been found very effective in reducing the adverse affects of drought stress. This study was conducted to examine the possible role of exogenous GB and SA application in improving the yield of hybrid sunflower (Helianthus annuus L.) under different irrigation regimes. There were three levels of irrigation, viz. control (normal irrigations), water stress at vegetative stage (irrigation missing at vegetative stage) and water stress at flowering stage (irrigation missing at flowering stage). GB and SA were applied exogenously at 100 and 0.724 mm, respectively, each at the vegetative and at the flowering stage. Control plants did not receive application of GB and SA. Water stress reduced the head diameter, number of achene, 1000‐achene weight, achene yield and oil yield. Nevertheless, exogenous GB and SA application significantly improved these attributes under water stress. However, drought stress increased the free leaf proline and GB, and were further increased by exogenous application of GB and SA. However, exogenous GB application at the flowering stage was more effective than other treatments. Oil contents were also reduced under water stress; however, GB and SA application could not ameliorate the negative effect of water stress on achene oil contents. The effects of water stress and foliar application of GB were more pronounced when applied at the flowering stage than at the vegetative stage. Moreover, exogenous GB application was only beneficial under stress conditions.
The optimum temperature for maize germination is between 25 and 28 °C. Poor and erratic germination at suboptimal temperature is the most important hindrance in its early sowing. This study was conducted to induce chilling tolerance in hybrid maize (Zea mays L.) by seed priming with salicylic acid (SA) and to unravel the background biochemical basis. For seed priming, maize hybrid (Hycorn 8288) seeds were soaked in 50, 100 and 150 ppm (mg l−1) aerated solutions of SA for 24 h and were dried back. Treated and untreated seeds were sown at 27 °C (optimal temperature) and at 15 °C (chilling stress) under controlled conditions. Performance of maize seedlings was hampered under chilling stress. But seed priming with SA improved the seedling emergence, root and shoot length, seedling fresh and dry weights, and leaf and root score considerably compared with control both at optimal and chilling temperatures. However, priming in 50 mg l−1 SA solution was more effective, followed by priming in 100 mg l−1 SA solution. Seed priming with SA improved the chilling tolerance in hybrid maize mainly by the activation of antioxidants (including catalase, superoxide dismutase and ascorbate peroxidase). Moreover, maintenance of high tissue water contents and reduced membrane permeability also contributed towards chilling tolerance.
A field study was conducted to investigate the influence of variable rates of application of N and P fertilizers in splits at various times on the growth and the seed and oil yields of canola (Brassica napus L.) during 1995–97. Rates of fertilizer application were 0 and 0 (F0), 60 and 0 (F1), 0 and 30 (F2), 60 and 30 (F3), 90 and 60 (F4) and 120 and 90 (F5) kg N ha−1 and kg P2O5 ha−1. All the P was applied at sowing while N was applied in splits, i.e. all at sowing, half at sowing and half with first irrigation, or half at sowing and half at flowering. The responses of growth, seed yield and components of yield were consistent in both years. Increasing the rate of fertilizer application from F4 (90/60 kg N/P2O5 ha−1) to F5 (120/90 kg N/P2O5 ha−1) increased the leaf area index (LAI) relative to the control and to lower rates of fertilizer application. For both crops, application of 90/60 kg N/P2O5 ha−1 significantly enhanced total dry matter (TDM) and seed yield. Seed yield increased mainly due to a greater number of pods per plant and seeds per seed‐pod. The time of fertilizer application did not significantly affect seed yield or components of yield in either season. Oil yield generally followed seed yield, increasing with increasing rate of fertilizer application up to 90/60 kg N/P2O5 ha−1. The maximum oil contents were obtained from the control. The results show that seed and oil yields of canola were maximized at the F4 (90/60 kg N/P2O5 ha−1) rate of application under the agro‐ecological conditions of Faisalabad, Pakistan.
Salinity is one of a major threat in harvesting good wheat stand on sustained basis. In this study, potential of seed priming techniques to improve the performance of wheat varieties (SARC‐1 and MH‐97) in a saline field was tested. For priming, wheat seeds were soaked in aerated solution of ascorbate (50 mg l−1; ascorbate priming), salicylic acid (50 mg l−1; salicylicate priming), kinetin (50 mg l−1; kinetin priming) and CaCl2 (50 mg l−1; osmopriming) for 12 h. For comparison, seeds were also soaked in simple water (hydropriming); in addition, untreated seeds were also taken as control. Seed priming treatments substantially improved the stand establishment; osmopriming (with CaCl2) was at the top however. Likewise maximum fertile tillers, grains per spike, 1000‐grain weight, grain yield and harvest index were observed in plants raised from seeds osmoprimed (with CaCl2) followed by ascorbate priming in both the varieties tested. As an index of salinity tolerance, seed priming treatments also improved the leaf K+ contents with simultaneous decrease in Na+ concentration, osmopriming being the best treatment. Similarly, maximum total phenolic contents, total soluble proteins (TSP), α‐amylase and protease activities were observed in osmoprimed (with CaCl2) seeds followed by ascorbate priming. Economic analysis also indicated that osmopriming is more viable with maximum net return and benefit‐to‐cost ratio. In conclusion, different seed priming treatments in wheat seeds improved the salinity tolerance nonetheless osmopriming (with CaCl2) was the most effective treatments to get higher grain yield and net return in both wheat varieties whereas kinetin was the least effective.
The increasing concern about the toxicity of synthetic herbicides has boosted the search for eco-friendly and sustainable weed management practices. Allelopathic control of weeds has received great attention in recent years as a possible alternative for weed management. Here, a two-year field study was conducted to explore the herbicidal potential of sorghum water extract alone and in combination with water extracts of other allelopathic plants: eucalyptus, sesame, sunflower, tobacco and brassica, against wild oat (Avena fatua) and canary grass (Phalaris minor), two noxious weeds of wheat fields. Water extracts were applied twice 30 and 40 days after sowing. Our results show that application of sorghum and sunflower extracts at 12 L ha −1 each was more effective than other combinations. This treatment reduced wild oat dry matter by 42-62%, and canary grass by 36-55%. Application of sorghum and sunflower at 6 L ha −1 each increased the wheat grain yield by 89% during the first year, and by 35% during the second year. Application of the synthetic herbicide isoproturon at 1000 g active ingredient ha −1 was more effective for weed inhibition and yield increase than allelopathic water extracts. Nevertheless, application of sorghum and sunflower at 6 L ha −1 was economically more viable than the other treatments, with the highest marginal rate of return of 2824%.
Though many soil components are involved in reactions whereby soluble fertilizer P is converted to less soluble and therefore less available forms, the relative influence of each phase in this process is not clearly defined. While both crystalline and amorphous Fe oxides readily react with P in noncalcareous soils, the contribution of these forms to P sorption in calcareous soils has received little attention. Thus, in this study, samples of 20 mainly calcareous Lebanese soils, ranging widely in relevant properties, were equilibrated for 6 d with 25 mL P solutions ranging from 12.5 to 62.5 mg kg−1. The data did not conform to the conventional Langmuir isotherm. Correlations and regression coefficients with actual P rentention from solution indicated an involvement of oxalate Fe, but there was no effect of CaCO3 particle size. Removal of Fe prior to equilibration had an inconsistent effect on P retention. The P behavior of calcareous soils may be modified by Fe oxides in such soils.
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