The increasing concern about the toxicity of synthetic herbicides has boosted the search for eco-friendly and sustainable weed management practices. Allelopathic control of weeds has received great attention in recent years as a possible alternative for weed management. Here, a two-year field study was conducted to explore the herbicidal potential of sorghum water extract alone and in combination with water extracts of other allelopathic plants: eucalyptus, sesame, sunflower, tobacco and brassica, against wild oat (Avena fatua) and canary grass (Phalaris minor), two noxious weeds of wheat fields. Water extracts were applied twice 30 and 40 days after sowing. Our results show that application of sorghum and sunflower extracts at 12 L ha −1 each was more effective than other combinations. This treatment reduced wild oat dry matter by 42-62%, and canary grass by 36-55%. Application of sorghum and sunflower at 6 L ha −1 each increased the wheat grain yield by 89% during the first year, and by 35% during the second year. Application of the synthetic herbicide isoproturon at 1000 g active ingredient ha −1 was more effective for weed inhibition and yield increase than allelopathic water extracts. Nevertheless, application of sorghum and sunflower at 6 L ha −1 was economically more viable than the other treatments, with the highest marginal rate of return of 2824%.
This contribution proposes an enzyme-assisted eco-friendly process for the extraction of non-extractable polyphenols (NEPPs) from black tea leftover (BTLO), an underutilized tea waste. BTLO hydrolyzed with various enzyme formulations was extracted using supercritical carbon dioxide and ethanol as co-solvent (SC-CO + EtOH). A conventional solvent extraction (CSE) was performed using EtOH + HO (80:20, v/v) for comparison purposes. The results revealed that hydrolysis of BTLO with 2.9% (w/w) kemzyme at 45 °C and pH 5.4 for 98 min improved the liberation of NEPPs offering 5-fold higher extract yield (g/100 g) as compared with non-treated BTLO. In vitro antioxidant evaluation and LC-MS characterization of extracts revealed the presence of phenolic acids (mainly caffeic and para-coumaric acid) of high antioxidant value. Scanning electron micrograph of the hydrolyzed BTLO samples indicated noteworthy changes in the ultrastructure of BTLO. Moreover, polyphenol extracts obtained by SC-CO + EtOH extraction were found to be cleaner and richer in polyphenols as compared to CSE. The devised enzyme-assisted SC-CO + EtOH extraction process in the present work can be explored as an effective biotechnological mean for the optimal recovery of antioxidant polyphenols. Graphical abstract Enzymatic pretreatment can effectively liberate non-extractable polyphenols (NEPPs) while hydrolyzing the cellulosic and hemicellulosic framework of black tea left overs (BTLO).
Enzyme assisted solvent extraction (EASE) of phenolic compounds from watermelon (C. lanatus) rind (WMR) was optimized using Response Surface Methodology (RSM) with Rotatable Central Composite Design (RCCD). Four variables each at five levels i.e. enzyme concentration (EC) 0.5-6.5 %, pH 6-9, temperature (T) 25-75 °C and treatment time (t) 30-90 min, were augmented to get optimal yield of polyphenols with maximum retained antioxidant potential. The polyphenol extracts obtained under optimum conditions were evaluated for their in-vitro antioxidant activities and characterized for individual phenolic profile by RP-HPLC-DAD. The results obtained indicated that optimized EASE enhanced the liberation of antioxidant phenolics up to 3 folds on fresh weight basis (FW) as compared to conventional solvent extraction (CSE), with substantial level of total phenolics (173.70 mg GAE/g FW), TEAC 279.96 mg TE/g FW and DPPH radical scavenging ability (IC50) 112.27 mg/mL. Chlorogenic acid (115.60-1611.04), Vanillic acid (26.13-2317.01) and Sinapic acid (113.01-241.12 μg/g) were major phenolic acid found in EASEx of WMR. Overall, it was concluded that EASE might be efficient and green technique to revalorize under-utilized WMR into potent antioxidant phenolic for their further application in food and nutraceutical industries.
Allelopathy has the potential to tackle the concerns associated with the indiscriminate use of synthetic herbicides. In search of a natural herbicide, the effects of mulberry (Morus alba) leaf water extracts on bermudagrass (Cynodon dactylon) and wheat (Triticum aestivum) were investigated. Four concentrations of extract (25, 50, 75, and 100%) were compared with a control (distilled water).The results revealed that the pregermination application of 100% mulberry leaf water extract resulted in the complete inhibition of bermudagrass and wheat germination. However, postemergence, two foliar sprays suppressed only the growth of bermudagrass and promoted wheat growth. The effect of the mulberry leaf water extract was concentration dependent. It is evident that the postemergence application of mulberry leaf water extract has the scope for use as natural herbicide for the control of bermudagrass and to promote the growth and yield of wheat.
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