Background: Periodontitis is an inflammatory disease in the periodontal tissue and causes progressive tissue damage. Tissue Inhibitor of Matrix Metalloproteinases-1 (TIMP-1) is directly responsible for the pathogenesis of periodontal diseases. Topical application of the metronidazole gel as a therapy for periodontal diseases can be used for additional therapy. This study aimed to analyze the effect of Metronidazole gel on periodontitis based on TIMP-1 levels in bacteria-induced periodontitis in rats. Methods: Twenty Sprague Dawley rats weighing 170-200 gram aged two months. Rats were divided into two groups: the control group and Metronidazole gel (Ti-es Metronidazole gel, 0.45 mg). Metronidazole gel was topically applied to the gingival sulcus. The gingival crevicular fluid sample was taken on day 3 with periopaper subjected to the Tissue Inhibitor of Matrix Metalloproteinase-1 level evaluation using the ELISA Method. Results: Normal reference Tissue Inhibitor of Matrix Metalloproteinase 1 levels gingival crevicular fluid 0.156-10 ng/ml. Tissue Inhibitor of Matrix Metalloproteinase 1 gingiva crevicular fluid in control and metronidazole gel was 1.34 ng/ ml and 3.57 ng /ml. There was a significant difference between control and metronidazole gel group (p<0.005) Conclusion: The Application of Metronidazole gel affects TIMP-1 levels in periodontitis.
Introduction: Alveolar bone resorption in periodontitis is caused by increased activation of osteoclasts, causing an imbalance of bone remodeling. Periodontitis treatment can be done mechanically with Scaling and Root Planing and accompanied by metronidazole. Antibiotics have long-term drawbacks and can cause allergies, thus requiring an alternative to natural topical drugs such as brown algae gel. This study aimed to to analyzed the effect of brown algae gel (sargassum sp) on the number of osteoclasts of alveolar bone in periodontitis rats. Methods: The research method was a True Experimental with a pretest-posttest-only control group design. Rats were divided into three groups; negative control (K-) was not given any treatment, positive control (K+) was given metronidazole ties plus 25% concentration, and the treatment group (KP) was given brown algae gel (Sargassum sp) 75% concentration. Data were analyzed by Friedman and Kruskal Wallis test. Results:There was a significant difference in the number of osteoclasts on days 8, 10, and 12, the decrease in the number of osteoclasts was higher in the brown algae treatment group than the negative control group (p=0.051). In the positive control group, there was a significant difference in the decrease of the number of osteoclasts compared to the brown algae treatment group (p=0.029). The number of osteoclasts was significantly different on day 12 (p=0.026).Conclusion: Adhering brown algae (Sargassum sp.) gel decreases osteoclasts in periodontitis rats.Keywords: bone remodeling; bone resorption; brown algae gel (sargassum sp.); osteoclasts; periodontitis.
Background: Curettage is an act of scraping the granulation tissue on the lateral gingival wall of the periodontal pocket. After curettage, a periodontal pack was applied to control bleeding and keep the wound area clean. The addition of iodine leaves as an alternative because it contains flavonoids that can help the wound healing process. This study aims to determine the effect of adding 10% iodine leaf extract gel on the curettage process.Method: A true experimental laboratory study with a post-test only control group design on 20 wistar rats. The periodontal pack group and the periodontal pack group were added with 10% iodine leaf extract gel. Curettage healing was seen from the mean number of fibroblasts using One-Way Anova analysis.Result: The study showed the mean number of fibroblasts on the 5th and 7th day periodontal pack groups were 35.20 and 49.60, while those in the periodontal pack group with the addition of iodine leaf extract gel on 5th and 7th days were 45.80 and 50.80, respectively. The One-Way Anova test showed p > 0.05, there was a significant difference in the mean number of fibroblasts between the study groups.Conclusion: The conclusion is that there is an effect of giving 10% iodine leaf extract gel on the number of fibroblasts in the healing process of wistar rat curettage.
Background: Background: The number of patients with periodontal disease in Indonesia is estimated to continue to increase yearly. 4.79% or 34614 people had healthy periodontal tissue, while 95.21% or 687715 people had unhealthy periodontal tissue. Clamshell waste containing chitosan can be utilized, considering that interest in sources of raw materials derived from waste is increasing. Chitosan has a dual function, which is osteoinductive because it contains growth factors that provide growth for osteoblast cells. Methods: A true experimental laboratory study with a post-test-only control group design was conducted on 30 Wistar rats and divided into two groups: the 0.2% chlorhexidine gluconate group and the chitosan group of scallop shells (Amusium Pleuronectes). The increase in the number of osteoblasts was compared and analyzed. Results: The results showed that the average in the control group on days 7,
Background: Aloe vera (AV) contains a variety of characteristic antibiotic and anti-inflammatory components that have potential advantages for periodontal disease therapy. Diagnostic and prognostic markers of periodontal disease through the activity of alkaline phosphatase (ALP) in gingival crevicular fluid. This study was conducted to evaluate the effect of giving AV gel on alkaline phosphatase levels in rats with bacteria-induced periodontitis Method: Thirty-six male Sprague dawley were healthy and active, weighing 170-200 grams, aged 2 months were distinguished into two groups: the control group of periodontitis given therapy Gel Metronidazole plus 25% and AV gel 99.03% as a treatment group. Aloe vera gel is administered topically in gingival sulcus at a dose of 300 mg/kg of rat weight. Samples were taken from gingival sulcus liquid on days 1, 2 and 3 using periopaper paper. Alkaline phosphatase levels are measured using a UV/Vis Spectrophotometer with a wavelength of 405 nm. Variant analysis is used to compare ALP levels in different groups Result: There was significant difference in alkaline phosphatase levels between the control group and the treatment group (p>0.05). In Sprague dawley rats with periodontitis, there was a significant decrease in ALP levels on days 1, 2 and 3 given Metronidazole gel plus 25% therapy and AV gel 99.03%. But the treatment group showed lower ALP levels than the control group Conclusion: This study revealed that the use of AV gel locally has an effect and can be as an adjunct therapy for periodontitis PENDAHULUANPeriodontitis merupakan salah satu masalah utama kesehatan mulut yang mempengaruhi kesehatan tubuh secara keseluruhan dengan prevalensi kasus 70% dari jumlah populasi global. 1 Penyakit inflamasi ini disebabkan oleh biofilm bakteri yang merusak jaringan penyangga gigi (ligamen periodontal, gingiva dan tulang alveolar) atau sering disebut jaringan periodonsium. 2 Aktivitas inflamasi yang terjadi di jaringan periodonsium dapat ditentukan melalui kadar alkaline phosphatase (ALP) dalam cairan sulkus gingiva (GCF). 3 Alkaline phosphatase adalah salah satu marker diagnostik pertama yang diidentifikasi dalam GCF dengan kadar lebih tinggi daripada di serum. 4 Alkaline Phosphatase tersimpan dalam granul spesifik dan vesikula sekretori neutrofil yang akan sekresikan dan bermigrasi ke daerah inflamasi. Analisis ALP pada cairan sulkus gingiva merupakan pendekatan yang paling praktis untuk analisis biokimia dari respon host pada penyakit periodontal. 5 Kadar ALP dapat diukur pada 0 jam sampai 14 hari dengan puncak kadar ALP pada hari ke 14 sebesar 56.75±11.50 IU/L. 4 Kenaikan kadar Alkaline Phosphatase secara signifikan pada cairan
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