This research aims to determine the content of secondary metabolite compounds and antibacterial activity of stem bark extract Melochia umbellate (Houtt) Stapf var. degrabrata. Samples of M. umbellate stem bark were extracted by meseration using methanol solvent. Separation and purification is done by partitioning, fractionation with chromatography, and recrystallization. Antibacterial activity test of hexane extract and third isolate from bark of M. umbellate was done by agar diffusion method against bacterium Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Phytochemical test results showed that the hexane extract of bark M. umbellate compounds containing alkaloids and triterpenoids. Isolates of compound D is a triterpenoid group compound, while FKa and FKb compounds are steroid group compounds. The hexane extract had the highest antibacterial activity against B. subtilis bacteria with inhibitory zone diameter 12,0 mm. Isolates of compound D have a weak inhibitory effect on all test bacteria. FKa compound isolates had the highest inhibition against B. subtilis and S. aureus bacteria with inhibitory zone diameters of 18,0 mm and 13,0 mm respectively. Whereas FKb compound isolates have the highest inhibitory effect against B. subtilis bacteria with inhibitory zone diameter 12,0 mm. The results of the test show that FKa compound from bark of M. umbellate has the potential to be antibacterial because the compound is able to inhibit bacterial growth with ˃ 14 mm obstacle zone, especially against B. subtilis bacteria.
Kedayan roots (Aristolochia papillfolia Ding hou) is phytomedicine that used conventionally by peoples as antidotum. This research aims to know antioxidant activity of ethyl acetate soluble fraction from kedayan roots extract. Fractionation method that used is solid-liquid extraction with ethyl acetat as solven so that produced fraction that dissolve in ethyl acetate, than fractionated again with liquid vacuum chromatography with eluent n-hexane and ethyl acetat with some proportion of each solven and produced fraction A (5:5), fraction B (4:5), fraction C (3:5), fraction D (2:5), fraction E (1:5), and fraction F (0:5). Antioxidant activity assay used DPPH (2,2-diphenyl-1picrylhydrazyl) as radical substance and determined by visible spectrophotometre. Than data analyzed with linier regression to know IC50. The result of fraction C from kedayan roots (Aristolochia pap illfolia Ding hou) in ethyl acetate fraction have antioxidant activity with IC50 139,11 ppm.
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