The Activity of Anti-Oxidide of Etil Acetate Soluble Fractions from Extract of Kedayan Roots (Aristolochia Papillfolia Ding Hou)

Abstract

Kedayan roots (Aristolochia papillfolia Ding hou) is phytomedicine that used conventionally by peoples as antidotum. This research aims to know antioxidant activity of ethyl acetate soluble fraction from kedayan roots extract. Fractionation method that used is solid-liquid extraction with ethyl acetat as solven so that produced fraction that dissolve in ethyl acetate, than fractionated again with liquid vacuum chromatography with eluent n-hexane and ethyl acetat with some proportion of each solven and produced fraction A (5:5), fraction B (4:5), fraction C (3:5), fraction D (2:5), fraction E (1:5), and fraction F (0:5). Antioxidant activity assay used DPPH (2,2-diphenyl-1picrylhydrazyl) as radical substance and determined by visible spectrophotometre. Than data analyzed with linier regression to know IC50. The result of fraction C from kedayan roots (Aristolochia pap illfolia Ding hou) in ethyl acetate fraction have antioxidant activity with IC50 139,11 ppm.