This study describes the antidiarrheal and antispasmodic activities of the crude extract of V. stocksii with possible mode of action explored. Materials and Methods Plant materialsAerial parts of V. stocksii (800 g) were obtained from Quetta Baluchistan and authenticated by Prof. Musaddar A Zaidi. A voucher specimen (VT-LF-09-02-57) was deposited at the herbarium located at the Department of Biological and Biomedical Sciences, Aga Khan University, Karachi, Pakistan. Preparation of crude extractPreparation of the crude extract was carried out as described previously (Gilani et al., 2005). The powdered material was soaked in 70% aqueous-methanol for three AbstractThis study was carried out to explore the mechanism underlying antidiarrheal and antispasmodic activities of Vincetoxicum stocksii. The crude extract of V. stocksii provided 12-24% protection from castor oil-induced diarrhea at the dose of 300-1,000 mg/kg, similar to loperamide. In isolated rabbit jejunum preparations, V. stocksii caused inhibition of the spontaneous and high K + (80 mM)-induced contractions, with respective EC50 values of 2.5 (1.7-3.9) and 1.0 mg/mL (0.6-1.4), similar to that caused by verapamil, suggesting the calcium channel blocking effect. Loperamide caused inhibition of spontaneous and high K + -induced contraction, with respective EC50 values of 8.6 (6.3-10.1) and 9.1 µM (7.3-12.8). The calcium channel blocking activity was further confirmed when pretreatment of the tissues with V. stocksii (1-3 mg/mL) caused a rightward displacement of the Ca ++ concentrations response curves, similar to that produced by verapamil; constructed in Ca ++ free medium. These data indicate that the crude extract of V. stocksii contains calcium channel blocking constituents that may possibly explain its medicinal use in hyperactive states of gut, such as, diarrhea and spasms. Article Info

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