Mst. Nasrin Akhter Banu scite author profile

Methylglyoxal (MG) is one of the aldehydes accumulated in plants under environmental stress. Cytosolic ascorbate peroxidase (cAPX) plays a key role in the protection of cells from oxidative damage by scavenging reactive oxygen species in higher plants. A cDNA encoding cAPX, named NtcAPX, was isolated from Nicotiana tabacum. We characterized recombinant NtcAPX (rNtcAPX) as a fusion protein with glutathione S‐transferase to investigate the effects of MG on APX. NtcAPX consists of 250 amino acids and has a deduced molecular mass of 27.5 kDa. The rNtcAPX showed a higher APX activity. MG treatments resulted in a reduction of APX activity and modifications of amino groups in rNtcAPX with increasing Km for ascorbate. On the contrary, neither NaCl nor cadmium reduced the activity of APX. The present study suggests that inhibition of APX is in part due to the modification of amino acids by MG. © 2012 Wiley Periodicals, Inc. J Biochem Mol Toxicol 26:315–321, 2012; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.21423

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The Effects of Methylglyoxal on Glutathione S-Transferase from Nicotiana tabacum

Hoque

Uraji

Banu

et al. 2010

Bioscience, Biotechnology, and Biochemistry

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Mst. Nasrin Akhter Banu

Exogenous proline and glycinebetaine increase NaCl-induced ascorbate–glutathione cycle enzyme activities, and proline improves salt tolerance more than glycinebetaine in tobacco Bright Yellow-2 suspension-cultured cells

Proline and glycinebetaine enhance antioxidant defense and methylglyoxal detoxification systems and reduce NaCl-induced damage in cultured tobacco cells

Proline and glycinebetaine induce antioxidant defense gene expression and suppress cell death in cultured tobacco cells under salt stress

Exogenous proline and glycinebetaine increase antioxidant enzyme activities and confer tolerance to cadmium stress in cultured tobacco cells

Proline and Glycinebetaine Ameliorated NaCl Stress via Scavenging of Hydrogen Peroxide and Methylglyoxal but Not Superoxide or Nitric Oxide in Tobacco Cultured Cells

Exogenous proline mitigates the detrimental effects of salt stress more than exogenous betaine by increasing antioxidant enzyme activities

Methylglyoxal inhibition of cytosolic ascorbate peroxidase from Nicotiana tabacum

The Effects of Methylglyoxal on Glutathione S-Transferase from Nicotiana tabacum

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