BackgroundAdaptations to different habitats across the globe and consequent genetic variation within rice have resulted in more than 120,000 diverse accessions including landraces, which are vital genetic resources for agronomic and quality traits. In India the rice landraces of the states West Bengal, Assam, Mizoram, Manipur and Nagaland are worthy candidates for genetic assessment. Keeping the above in view, the present study was conducted with the aim to (i) calculate the genetic distances among the accessions of 83 landraces collected from these states along with 8 check accessions (total 91 accessions) using 23 previously mapped SSR markers and (ii) examine the population structure among the accessions using model-based clustering approach.ResultsAmong the 91 accessions, 182 alleles were identified which included 51 rare and 27 null alleles. The average PIC value was 0.7467/marker. The non-aromatic landraces from West Bengal was most diverse with 154 alleles and an average PIC value of 0.8005/marker, followed by the aromatic landraces from West Bengal with 118 alleles and an average PIC value of 0.6524/marker, while the landraces from North East ranked third with 113 alleles and an average PIC value of 0.5745/marker. In the dendrogram distinct clusters consisting of predominantly aromatic landraces and predominantly North East Indian landraces were observed. The non-aromatic landraces from West Bengal were interspersed within these two clusters. The accessions were moderately structured, showing four sub-populations (A-D) with an Fst value of 0.398, 0.364, 0.206 and 0.281, respectively. The assigned clustering of accessions was well in agreement in both distance-based and model-based approaches.ConclusionsEach of the accessions could be identified unequivocally by the SSR profiles. Genetically the non aromatic landraces from West Bengal were most diverse followed by the aromatic landraces from the same state. The North Eastern accessions ranked third. Further, grouping of accessions based on their agronomic traits may serve as a resource for future studies, leading to the improvement of rice. Moreover in-situ preservation of the landraces is also a means of protection of biodiversity and cultural heritage.

Growth environment and plant nutrition are two important factors influencing growth, yield and quality of aromatic rice (Oryza sativa L.). The present study was conducted at Kalyani, India to determine the effect of two planting dates and four fertilizer levels on different aromatic rice cultivars during the dry seasons of 1995/96 and 1996/97, while nine cultivars were evaluated during the wet seasons of 1996 and 1997. Thermal and photoperiodic conditions significantly influenced the vegetative (leaf area index and light extinction co-efficient) and reproductive (filled spikelets/panicle) growth of the crop. Delayed planting (23 February) significantly reduced the grain yield by 0·88 t/ha, amylose content by 0·5% and duration by 10 days; but increased the summed heliothermal units (17806 v. 18505). Thus, the cultivars became less efficient (27%) in heat use with delay in planting from 2 to 23 February. Relative availability of NH4+-N from urea and Azolla influenced the crop growth (leaf area index [LAI], tiller production and leaf chlorophyll content) and nutrient uptake. Supply of inorganic N either alone or in conjunction with Azolla significantly increased grain yield (18–41%) and protein content (0·1–0·7%) over 15 t/ha of Azolla alone. However, combined application of Azolla and urea lowered the amylose content below that achieved by application of either substance alone. Correlation studies among quality attributes indicated that long-grained varieties had lower head rice recovery (r=−0·69) due to more breakage during milling and greater test weight (r=0·93).

Aims: The present study describes the detection and quantification of the Sarocladium oryzae metabolites, helvolic acid and cerulenin in extracts of rice grains collected from plants infected with sheath rot. It also describes the phytotoxicity of these metabolites on rice seedlings. Methods and Results: Helvolic acid and cerulenin in sheath rot-infected rice grains were detected using thin layer chromatography (TLC) and nuclear magnetic resonance (NMR) analyses. On the TLC plates helvolic acid and cerulenin moved as brownish yellow spots and showed R F values of 0AE61 and 0AE49, respectively. A standard assay curve was developed on the basis of selective toxicity of helvolic acid towards Calvibacter michiganensis ATCC 2140 and cerulenin towards Candida albicans 1150. The amounts of helvolic acid and cerulenin on the basis of standard assay curve were 2AE2 and 1AE75 lg g )1 of infected seeds. Treatment of IR 36 rice seedlings with metabolites induced chlorosis and reduced shoot length by 20%, root length by 30% and root number by 7% relative to control. Conclusions: Helvolic acid and cerulenin were detected in infected rice grains and these metabolites induced chlorosis and reduced the seed viability and seedling health of rice. Significance and Impact of the Study: Antimicrobial and phytotoxic metabolites, helvolic acid and cerulenin are present in infected grains and reduce the seed viability and seedling health. These metabolites may increase the pathogenic potential and survival of S. oryzae in rice seed by competing with other seed-borne fungi.

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