Rhizospheric actinobacteria associated to organic cultivation are essential in nutrient cycling and plant growth promotion. The aim of thi s study was to isolate and select in vitro actinobacteria displaying multiple plant growth promoting traits associated with the rhizosphere of sugarcane undergoing organic management. The isolates were evaluated regarding their ability to produce plant growth promoting traits. Of the 21 isolates, 57% produced at least one of the evaluated traits. Isolates ABC92 and ABC32 produced 60.28 and 55.36 μg mL -1 of fitohormone in 21 days, whereas isolates ANC48 and ANU34 were the best solubilizers, solubilizing 8.93 and 8.92 μg mL -1 phosphate. A total of 29% of the microorganisms were able to grow in nitrogen-free media and 24% were ammonia producers. Isolates ABC31, ANC48 and ANU49 were able to inhibit Fusarium moniliforme growth. All actinobacteria identified in this study belonged to the Streptomyces genus and presented potential as plant growth promoting agents.