The formation of atherosclerotic plaques is the root cause of various cardiovascular diseases (CVDs). Effective CVD interventions thus call for precise identification of the plaques to aid clinical assessment, diagnosis, and treatment of such diseases. In this study, we introduce a dual-target sequentially activated fluorescence reporting system, termed in-sequence highspecificity dual-reporter unlocking (iSHERLOCK), to precisely identify the atherosclerotic plaques in vivo and ex vivo. ISHERLOCK was achieved by creating a threein-one fluorescent probe that permits highly specific and sensitive detection of lipid droplets and hypochlorous acid via "off-on" and ratiometric readouts, respectively. Based on this format, the upregulated lipid accumulation and oxidative stress-the two hallmarks of atherosclerosis (AS)-were specifically measured in the atherosclerotic plaques, breaking through the barrier of precise tissue biopsy of AS and thus aiding effective CVD stewardship.
Bioanalytics. In their Research Article (e202204518), Dingbin Liu et al. designed a dual‐reporter unlocking platform, termed iSHERLOCK, to detect lipid droplets and HClO via fluorescent readouts. Based on iSHERLOCK, two hallmarks of atherosclerosis were quantitatively measured.
The formation of atherosclerotic plaques is the root cause of various cardiovascular diseases (CVDs). Effective CVD interventions thus call for precise identification of the plaques to aid clinical assessment, diagnosis, and treatment of such diseases. In this study, we introduce a dual‐target sequentially activated fluorescence reporting system, termed in‐sequence high‐specificity dual‐reporter unlocking (iSHERLOCK), to precisely identify the atherosclerotic plaques in vivo and ex vivo. ISHERLOCK was achieved by creating a three‐in‐one fluorescent probe that permits highly specific and sensitive detection of lipid droplets and hypochlorous acid via “off‐on” and ratiometric readouts, respectively. Based on this format, the upregulated lipid accumulation and oxidative stress—the two hallmarks of atherosclerosis (AS)—were specifically measured in the atherosclerotic plaques, breaking through the barrier of precise tissue biopsy of AS and thus aiding effective CVD stewardship.
Bioanalytik. Dingbin Liu et al. beschreiben in ihrem Forschungsartikel (e202204518) eine Reporterplattform, genannt iSHERLOCK, für den Fluoreszenznachweis von Lipidtröpfchen und HClO. Mittels iSHERLOCK wurden zwei Atherosklerosemarker quantitativ gemessen.
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